Genetic markers and characterization studies of Centella asiatica


Citation

Wong S.M., . and Daud S.K., . and Fadzillah N.M., . and Marziah M., . (2001) Genetic markers and characterization studies of Centella asiatica. [Proceedings Paper]

Abstract

DNA extraction protocols with minor modification have been used to get high purity of genomic DNA and reproducible results. Two types of PCR-based DNA markers LP-RAPD Long Primer-Randomly Amplify Polymorphic DNA and AFLP Amplified Fragment Length Polymorphism were applied to identify and characterize 12 accessions of C. asiatica. In LP-RAPD study optimum concentration and conditions for the protocal have been obtained. Amplification using long primers that each comprosing of 18 to 24 bases will generates 6 to 30 DNA fingerprints. Six out of ten single primers ERICIRERIC2 PehA3 PehA6 Puc/M13F BOXIR were selected for further LP-RADP analysis. In AFLP study the screening of 64 primer combinations on CA3 and CA6 are still in the progress.


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Abstract

DNA extraction protocols with minor modification have been used to get high purity of genomic DNA and reproducible results. Two types of PCR-based DNA markers LP-RAPD Long Primer-Randomly Amplify Polymorphic DNA and AFLP Amplified Fragment Length Polymorphism were applied to identify and characterize 12 accessions of C. asiatica. In LP-RAPD study optimum concentration and conditions for the protocal have been obtained. Amplification using long primers that each comprosing of 18 to 24 bases will generates 6 to 30 DNA fingerprints. Six out of ten single primers ERICIRERIC2 PehA3 PehA6 Puc/M13F BOXIR were selected for further LP-RADP analysis. In AFLP study the screening of 64 primer combinations on CA3 and CA6 are still in the progress.

Additional Metadata

[error in script]
Item Type: Proceedings Paper
Additional Information: Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QD415 A1N277 2000
AGROVOC Term: APIACEAE
AGROVOC Term: DNA
AGROVOC Term: EXTRACTS
AGROVOC Term: CHROMOSOMES
AGROVOC Term: MALAYSIA
Geographical Term: MALAYSIA
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:27
URI: http://webagris.upm.edu.my/id/eprint/15938

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