Insecticidal activity of leaf tissue and in vitro culture extracts of Azadirachta indica A. Juss Neem against the larvae of flour beetles Tribolium castaneum

Sanimah S., . and Radzali M., . and Johari R, . and Mohd Arif S., . (2001) Insecticidal activity of leaf tissue and in vitro culture extracts of Azadirachta indica A. Juss Neem against the larvae of flour beetles Tribolium castaneum. [Proceedings Paper]

Crude ethanol methanol and chloroform extracts of leaf tissues calluses and cell-suspensions of Azadirachta indica A. Juss neem were bioassayed for toxicity to larvae of flour beetles Tribolium castaneum. Experiments have been done by feeding the larvae with artificial breeding medium ABM containing the various concentrations of the extracts. Bioasay of the larvae of T. castaneum revealed that the organic fraction of ethanol methanol and chloroform obtained from leaf tissues calluses and cell-supension of A. indica were capable of inhibiting he growth of the larvae although the mortality was not observed throughout the experiment. This result suggested that the bioactive compounds that might be responsible for the inhibition were capable of inducing the multiple effects resulting in interference in the growth of the larvae. Methanol fraction from leaf tissues of A. indica showed higher inhibition activity compared to the other fraction from leaf tissues calluses and cell-supensions. Treatment with 125pp extract of leaf tissues resulted in 50 inhibition of the growth compared to the control.

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Abstract

Crude ethanol methanol and chloroform extracts of leaf tissues calluses and cell-suspensions of Azadirachta indica A. Juss neem were bioassayed for toxicity to larvae of flour beetles Tribolium castaneum. Experiments have been done by feeding the larvae with artificial breeding medium ABM containing the various concentrations of the extracts. Bioasay of the larvae of T. castaneum revealed that the organic fraction of ethanol methanol and chloroform obtained from leaf tissues calluses and cell-supension of A. indica were capable of inhibiting he growth of the larvae although the mortality was not observed throughout the experiment. This result suggested that the bioactive compounds that might be responsible for the inhibition were capable of inducing the multiple effects resulting in interference in the growth of the larvae. Methanol fraction from leaf tissues of A. indica showed higher inhibition activity compared to the other fraction from leaf tissues calluses and cell-supensions. Treatment with 125pp extract of leaf tissues resulted in 50 inhibition of the growth compared to the control.

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Item Type:	Proceedings Paper
Additional Information:	Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QD415 A1N277 2000
AGROVOC Term:	AZADIRACHTA INDICA
AGROVOC Term:	LARVAE
AGROVOC Term:	COLEOPTERA
AGROVOC Term:	TRIBOLIUM CASTANEUM
AGROVOC Term:	IN VITRO
AGROVOC Term:	CALLUS
AGROVOC Term:	EXTRACTS
AGROVOC Term:	PESTICIDAL PROPERTIES
AGROVOC Term:	LEAVES
AGROVOC Term:	MALAYSIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15939

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