Shootlets formation of Eurycoma longifolia through direct regeneration techniques

Sobri H., . and Marziah M., . (2001) Shootlets formation of Eurycoma longifolia through direct regeneration techniques. [Proceedings Paper]

Eurycoma longifolia Tongkat Ali belongs to the family of the Simaroubaceae. It is a single-stemmed slow-growing understorey tree of the rainforests of Southeast Asia. It is used throughout the region as a herbal medicine and tonic. Chemical compounds such as quassinoids sitosterol stigmasterol melianone niloticin scopoletin eurycomanol eurylene hispidone campesterol and several others have been characterized in leaves rots and stems of E. longifolia. Most of the active ingredients are concentrated in the taproot of the plant. Tongkat Ali planted at Plant Genetic Center PGC in Universiti Putra Malaysia UPM was employed in carrying out most of the experiments this study. Some plant meterials from Pulau Langkawi and Hutan Belum Perak Malaysia were also used. DKW medium supplemented with 1 mg/L and 2 mg/L BAP induce the highest percentage of shoots formation 75 and followed by 3 mg/L BAP 50 and 4 mg/L BAP 25. The same phenomenon is noticed in treatment with WPM. The effect of media composition on the direct regeneration studies show that MS medium is not suitable for higher shoot formation compared to DKW and WPM medium.

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Abstract

Eurycoma longifolia Tongkat Ali belongs to the family of the Simaroubaceae. It is a single-stemmed slow-growing understorey tree of the rainforests of Southeast Asia. It is used throughout the region as a herbal medicine and tonic. Chemical compounds such as quassinoids sitosterol stigmasterol melianone niloticin scopoletin eurycomanol eurylene hispidone campesterol and several others have been characterized in leaves rots and stems of E. longifolia. Most of the active ingredients are concentrated in the taproot of the plant. Tongkat Ali planted at Plant Genetic Center PGC in Universiti Putra Malaysia UPM was employed in carrying out most of the experiments this study. Some plant meterials from Pulau Langkawi and Hutan Belum Perak Malaysia were also used. DKW medium supplemented with 1 mg/L and 2 mg/L BAP induce the highest percentage of shoots formation 75 and followed by 3 mg/L BAP 50 and 4 mg/L BAP 25. The same phenomenon is noticed in treatment with WPM. The effect of media composition on the direct regeneration studies show that MS medium is not suitable for higher shoot formation compared to DKW and WPM medium.

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Item Type:	Proceedings Paper
Additional Information:	Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QD415 A1N277 2000
AGROVOC Term:	SIMAROUBACEAE
AGROVOC Term:	RAIN FORESTS
AGROVOC Term:	DRUG PLANTS
AGROVOC Term:	SHOOTS
AGROVOC Term:	REGENERATION
AGROVOC Term:	TISSUE CULTURE
AGROVOC Term:	MALAYSIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/15940

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