Citation
Ling A.P.K., . and Marziah M., . and Tan S.E., . (2001) Triterpenoids distribution in whole plant and callus cultures of Centella asiatica accessions. [Proceedings Paper]
Abstract
Centella asiatica has recently become popular due to its pharmaceutical and cosmetic properties. Recent studies have confirmed the efficacy of the plant and its extract primarily in treatment of skin injuries and disease including wounds burns ulcers leprosy and scleroderma. This study aimed in determining the distribution of triterpenoids in different plants parts of Centella asiatica accessions namely the leaf petiole stolon and root as well as leaf derived callus of CA01 using Thin Layer Chromatography TLC analysis. TLC analysis using ethyl acetate:methanol:water 8:2:1 as the solvent system showed that the 4 targeted compounds madecassoside asiaticoside madecassic acid and asiatic acid were detected in the fresh leaves. For fresh petioles stolons as well as roots only asiatic acid and madecassic acid were detected in all the accessions. Among all the plant parts analyzed phytochemicals were hardly detectable in roots even with the increase of the spotted volume from 20 microL and 30 microL. Findings from this study suggested that although there are 12 accessions of Pegaga which can be differentiated morphologically no variation can be detected in terms of the presence of the targeted phytochemicals. However from the size of the spots obtained in the chromatogram it showed that the concentrations or the quantity of the phytochemicals present were varied among the 12 accessions. This study also revealed that to detect the presence of phytochemicals in callus cultures through TLC a high amount of samples was needed. Asiatic acid and madecassic acid can only be detected clearly with 8 g of fresh callus samples however the spots of asiaticoside as well as madecassoside detected were not so clear. This could be due to the concentration of the 2 glycosides was too minute to be detected using TLC. None of the 4 targeted phytochemicals were detected in 2 g and 5 g of the samples.
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Abstract
Centella asiatica has recently become popular due to its pharmaceutical and cosmetic properties. Recent studies have confirmed the efficacy of the plant and its extract primarily in treatment of skin injuries and disease including wounds burns ulcers leprosy and scleroderma. This study aimed in determining the distribution of triterpenoids in different plants parts of Centella asiatica accessions namely the leaf petiole stolon and root as well as leaf derived callus of CA01 using Thin Layer Chromatography TLC analysis. TLC analysis using ethyl acetate:methanol:water 8:2:1 as the solvent system showed that the 4 targeted compounds madecassoside asiaticoside madecassic acid and asiatic acid were detected in the fresh leaves. For fresh petioles stolons as well as roots only asiatic acid and madecassic acid were detected in all the accessions. Among all the plant parts analyzed phytochemicals were hardly detectable in roots even with the increase of the spotted volume from 20 microL and 30 microL. Findings from this study suggested that although there are 12 accessions of Pegaga which can be differentiated morphologically no variation can be detected in terms of the presence of the targeted phytochemicals. However from the size of the spots obtained in the chromatogram it showed that the concentrations or the quantity of the phytochemicals present were varied among the 12 accessions. This study also revealed that to detect the presence of phytochemicals in callus cultures through TLC a high amount of samples was needed. Asiatic acid and madecassic acid can only be detected clearly with 8 g of fresh callus samples however the spots of asiaticoside as well as madecassoside detected were not so clear. This could be due to the concentration of the 2 glycosides was too minute to be detected using TLC. None of the 4 targeted phytochemicals were detected in 2 g and 5 g of the samples.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. QD415 A1N277 2000 |
| AGROVOC Term: | UMBELLIFLORAE |
| AGROVOC Term: | DRUG PLANTS |
| AGROVOC Term: | TRITERPENOIDS |
| AGROVOC Term: | TISSUE CULTURE |
| AGROVOC Term: | CHROMATOGRAPHY |
| AGROVOC Term: | EXTRACTS |
| AGROVOC Term: | MALAYSIA |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:27 |
| URI: | http://webagris.upm.edu.my/id/eprint/15941 |
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