Analyses of crude palm oil carotenoids by HPLC with three-dimensional diode array detection

Tay B.Y.P., . and Choo Y.M., . (2001) Analyses of crude palm oil carotenoids by HPLC with three-dimensional diode array detection. [Proceedings Paper]

The high performance liquid chromatography HPLC variable wavelength UV-visible detector was among the first method used for palm carotene analysis. However this method is inflexible as it requires multiple injection for one sample. This is because different carotenes have different absorption maxima. Furthermore identification is tedious individual pure carotene is collected and its spectrum recorded on a UV-visible spectrophotometer. These limitations are solved by using a HPLC-photodiode array system PDA. The HPLC-PDA is able to perform rapid on-line spectra scan without disturbing the chromatographic process. This means that only one sample injection is needed at any chosen wavelength. The chromatographic and spectral data at other wavelengths can be retrieved after the analyses. This paper gives a guideline on establishing carotene profile for crude palm oil using a three-dimensional PDA detector. Eleven types of carotenes: phytoene phytofluene lycopene alpha-zeacarotene beta-zeacarotene neurosporene delta-carotene gamma-carotene epsilon-carotene cis-alpha-carotene alpha-carotene and beta-carotene were detected for this sample. However the 3-D chromatogram showed that there are few other peaks hidden behind the alpha-carotene and beta-carotene peaks. The UV spectra for these hidden peaks suggest them to be isomers of these carotenes. Quantification for the carotenes was by area normalization. The HPLC-PDA can be used as a routine tool for fast but tentative analyses of palm carotenoids.

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Abstract

The high performance liquid chromatography HPLC variable wavelength UV-visible detector was among the first method used for palm carotene analysis. However this method is inflexible as it requires multiple injection for one sample. This is because different carotenes have different absorption maxima. Furthermore identification is tedious individual pure carotene is collected and its spectrum recorded on a UV-visible spectrophotometer. These limitations are solved by using a HPLC-photodiode array system PDA. The HPLC-PDA is able to perform rapid on-line spectra scan without disturbing the chromatographic process. This means that only one sample injection is needed at any chosen wavelength. The chromatographic and spectral data at other wavelengths can be retrieved after the analyses. This paper gives a guideline on establishing carotene profile for crude palm oil using a three-dimensional PDA detector. Eleven types of carotenes: phytoene phytofluene lycopene alpha-zeacarotene beta-zeacarotene neurosporene delta-carotene gamma-carotene epsilon-carotene cis-alpha-carotene alpha-carotene and beta-carotene were detected for this sample. However the 3-D chromatogram showed that there are few other peaks hidden behind the alpha-carotene and beta-carotene peaks. The UV spectra for these hidden peaks suggest them to be isomers of these carotenes. Quantification for the carotenes was by area normalization. The HPLC-PDA can be used as a routine tool for fast but tentative analyses of palm carotenoids.

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Item Type:	Proceedings Paper
Additional Information:	Available at Perpustakaan Sultan Abdul Samad Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia. TP684 P3N277 2000 Call Number
AGROVOC Term:	ELAEIS GUINEENSIS
AGROVOC Term:	PALM OILS
AGROVOC Term:	CHEMICAL COMPOSITION
AGROVOC Term:	HPLC
AGROVOC Term:	ULTRAVIOLET SPECTROPHOTOMETRY
AGROVOC Term:	CAROTENOIDS
AGROVOC Term:	QUALITATIVE ANALYSIS
AGROVOC Term:	MALAYSIA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/16059

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