Antioxidant effect of vitamin c and polyphenol in testis and spermatozoa.

Park M.Y., . and Kim C.K., . and Chang Y.M., . and Ryu J.W., . and Lee J.H., . and Chung Y.C., . and Pang M.Y., . (2004) Antioxidant effect of vitamin c and polyphenol in testis and spermatozoa. [Proceedings Paper]

Oxidative stress due to excessive production of reactive oxygen species ROS has been associated with defective sperm function and infertility. ROS form in the tissue injury in liver brain lung testis and other organs. Organisms have developed many defense mechanisms to protect themselves from injuries by ROS. The small molecule antioxidants such as Vit C and Vit E are able to interact with oxidizing radicals directly. Also tea catechin and polyphenols have been found to be efficient scavengers of free radicals in a number of in vitro systems. This study was conducted to determine whether treatment with Vit C and polyphenol protects porcine spermatozoa by inhibiting ROS generation induced by xanthine-xanthine oxidase system X-XO changes spermatogenesis and antioxidant activity of testis in Vit C deficiency guinea pigs. The first model received Vit C-free diet with low level Vit C water in orally Vit C 50. The second model was dieted with rich Vit C 100 and the third model received Vit C-free diet. The extended porcine semen served as a control the extended semen was treated with X-XO and the treatment semen supplemented with Vit C 0.0025 and polyphenol 0.01. The percentage of motile spermatozoa was evaluated by computer-assisted sperm analysis CASA and viability hypoosmotic swelling test HOST and chlorteracycline/Hochest 258 CTC staining was evaluated as parameters of sperm function test. The increased round spermatid model 1 397.89 : 348.72 model 2 255.90 : 430.87 model 3 431.90 : 345.61 : 400.88 x 10E6 spermatids /g testis tissue and glutathioneGSH level model 1 14.89 : 17.18 model 2 10.18 : 9.78 model 313.59 : 15.11 : 14.00 mM GSH/g testis tissue in the treatment polyphenol in vivo has shown in this investigation. The low level of lipid peroxidation in three models has shown model 1 42.32 : 21.70 model 2 20.22 : 12.57 model 3 8.22 : 7.46 : 8.74 muM MDA/g testis tissue. There was no significant difference total antioxidant status in the testis liver and serum of guinea pig. The porcine sperm motility was significantly increased by the addition of Vit C and polyphenol it could not be reached to the percentage of catalase addition. There was no significant difference between the treatment groups in liquid semen storage effect by the addition of Vit C and polyphenol. The data suggest that the addition of Vit C and polyphenol might reduce oxidative stress by ROS generation was sufficient to overcome by these antioxidants.

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Abstract

Oxidative stress due to excessive production of reactive oxygen species ROS has been associated with defective sperm function and infertility. ROS form in the tissue injury in liver brain lung testis and other organs. Organisms have developed many defense mechanisms to protect themselves from injuries by ROS. The small molecule antioxidants such as Vit C and Vit E are able to interact with oxidizing radicals directly. Also tea catechin and polyphenols have been found to be efficient scavengers of free radicals in a number of in vitro systems. This study was conducted to determine whether treatment with Vit C and polyphenol protects porcine spermatozoa by inhibiting ROS generation induced by xanthine-xanthine oxidase system X-XO changes spermatogenesis and antioxidant activity of testis in Vit C deficiency guinea pigs. The first model received Vit C-free diet with low level Vit C water in orally Vit C 50. The second model was dieted with rich Vit C 100 and the third model received Vit C-free diet. The extended porcine semen served as a control the extended semen was treated with X-XO and the treatment semen supplemented with Vit C 0.0025 and polyphenol 0.01. The percentage of motile spermatozoa was evaluated by computer-assisted sperm analysis CASA and viability hypoosmotic swelling test HOST and chlorteracycline/Hochest 258 CTC staining was evaluated as parameters of sperm function test. The increased round spermatid model 1 397.89 : 348.72 model 2 255.90 : 430.87 model 3 431.90 : 345.61 : 400.88 x 10E6 spermatids /g testis tissue and glutathioneGSH level model 1 14.89 : 17.18 model 2 10.18 : 9.78 model 313.59 : 15.11 : 14.00 mM GSH/g testis tissue in the treatment polyphenol in vivo has shown in this investigation. The low level of lipid peroxidation in three models has shown model 1 42.32 : 21.70 model 2 20.22 : 12.57 model 3 8.22 : 7.46 : 8.74 muM MDA/g testis tissue. There was no significant difference total antioxidant status in the testis liver and serum of guinea pig. The porcine sperm motility was significantly increased by the addition of Vit C and polyphenol it could not be reached to the percentage of catalase addition. There was no significant difference between the treatment groups in liquid semen storage effect by the addition of Vit C and polyphenol. The data suggest that the addition of Vit C and polyphenol might reduce oxidative stress by ROS generation was sufficient to overcome by these antioxidants.

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Item Type:	Proceedings Paper
Additional Information:	Summary only En
AGROVOC Term:	GUINEA PIGS
AGROVOC Term:	SPERMATOZOA
AGROVOC Term:	TESTES
AGROVOC Term:	SPERMATOGENESIS
AGROVOC Term:	OXIDATION
AGROVOC Term:	STRESS
AGROVOC Term:	ANTIOXIDANTS
AGROVOC Term:	ASCORBIC ACID
AGROVOC Term:	POLYPHENOLS
AGROVOC Term:	LIPID PEROXIDATION
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:27
URI:	http://webagris.upm.edu.my/id/eprint/16385

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