Prevalence and intensity of larvae of the Genus Anisakis sensu lato (Nematoda, Anisakidae) in Bigeye Scad, Selar crumenophthalmus (Bloch 1793), from the Indian Ocean off Java, Indonesia

Rizka Fauziana Syarifah and Murwantoko, Murwantoko and Setyobudi, Eko (2023) Prevalence and intensity of larvae of the Genus Anisakis sensu lato (Nematoda, Anisakidae) in Bigeye Scad, Selar crumenophthalmus (Bloch 1793), from the Indian Ocean off Java, Indonesia. Journal of Sustainability Science and Management (Malaysia), 36. pp. 192-202. ISSN 2073-3720

This research investigates the prevalence and mean intensity of Anisakis larvae on the bigeye scad, Selar crumenophthalmus (Bloch, 1793), along the Indian Ocean off Java. In total, 498 fish samples were collected from four locations: the Indian Ocean off East Java, Yogyakarta Special Territory, Central Java, and West Java. Each fish sample was measured for length and weight, then examined for Anisakis larvae in the abdominal cavity, liver, gonads, digestive tract, and muscle. Morphological characterisation was done using scanning electron microscope (SEM). The ITS rDNA region was amplified using PCR, then used to perform RFLP for identification and sequenced to form a phylogenetic tree. The results demonstrated that the bigeye scad was vulnerable to infection by Anisakis nematodes with varying prevalence and mean intensity at each location. The bigeye scad from the Indian Ocean off East Java had the highest prevalence and mean intensity (P = 75.44 %; MI = 10.38 larvae per-host), while from West Java had the lowest prevalence (P = 1.45 %; MI = 3.33 larvae per-host). The prevalence of Anisakis in the bigeye scad tends to decrease as one moves to the west from the sampling locations. Most larvae were found in the abdominal cavity (70 %–100 %), whereas a relatively low prevalence was found in other organs. The direct sequencing in the ITS rDNA region showed that Anisakis larvae found in the bigeye scad were A. typica (Diesing, 1860), which was indicated as A. typica var. indonesiensis (A. typica sp. B). Anisakis infection can be utilised as a biological indicator for movement patterns and fish stock differentiation.

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Abstract

This research investigates the prevalence and mean intensity of Anisakis larvae on the bigeye scad, Selar crumenophthalmus (Bloch, 1793), along the Indian Ocean off Java. In total, 498 fish samples were collected from four locations: the Indian Ocean off East Java, Yogyakarta Special Territory, Central Java, and West Java. Each fish sample was measured for length and weight, then examined for Anisakis larvae in the abdominal cavity, liver, gonads, digestive tract, and muscle. Morphological characterisation was done using scanning electron microscope (SEM). The ITS rDNA region was amplified using PCR, then used to perform RFLP for identification and sequenced to form a phylogenetic tree. The results demonstrated that the bigeye scad was vulnerable to infection by Anisakis nematodes with varying prevalence and mean intensity at each location. The bigeye scad from the Indian Ocean off East Java had the highest prevalence and mean intensity (P = 75.44 %; MI = 10.38 larvae per-host), while from West Java had the lowest prevalence (P = 1.45 %; MI = 3.33 larvae per-host). The prevalence of Anisakis in the bigeye scad tends to decrease as one moves to the west from the sampling locations. Most larvae were found in the abdominal cavity (70 %–100 %), whereas a relatively low prevalence was found in other organs. The direct sequencing in the ITS rDNA region showed that Anisakis larvae found in the bigeye scad were A. typica (Diesing, 1860), which was indicated as A. typica var. indonesiensis (A. typica sp. B). Anisakis infection can be utilised as a biological indicator for movement patterns and fish stock differentiation.

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Item Type:	Article
AGROVOC Term:	Selar crumenophthalmus
AGROVOC Term:	Anisakis
AGROVOC Term:	larvae
AGROVOC Term:	parasitology
AGROVOC Term:	research
AGROVOC Term:	biological sampling
AGROVOC Term:	Data analysis
AGROVOC Term:	fisheries biology
AGROVOC Term:	biological indicators
AGROVOC Term:	population distribution
Geographical Term:	Indonesia
Uncontrolled Keywords:	Anisakid, intensity, Indian Ocean, prevalence, pelagic
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	20 Mar 2025 04:25
Last Modified:	20 Mar 2025 04:25
URI:	http://webagris.upm.edu.my/id/eprint/1641

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