Phalaenopsis and protoplast culture


Citation

Ichihashi S., . and Shigemura S., . (2005) Phalaenopsis and protoplast culture. [Proceedings Paper]

Abstract

Plantlets were obtained by culture of orchid protoplasts. Callus of Phalaenopsis Wedding March was pre-cultured on sucrose-free medium for 3 weeks. Protoplasts were isolated from the pre-cultured callus. About 10 of the protoplasts cultured in sorbitol or maltose containing media divided within 4 weeks. Eleven months were required for Phalaenopsis protoplasts to produce PLB about 2-3 mm in size. About 100 PLBs were obtained from 1.5 x 10 protoplasts. These PLB when transplanted onto Kyoto medium developed into plantlets which were transplanted and successfully grown under greenhouse conditions.


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Abstract

Plantlets were obtained by culture of orchid protoplasts. Callus of Phalaenopsis Wedding March was pre-cultured on sucrose-free medium for 3 weeks. Protoplasts were isolated from the pre-cultured callus. About 10 of the protoplasts cultured in sorbitol or maltose containing media divided within 4 weeks. Eleven months were required for Phalaenopsis protoplasts to produce PLB about 2-3 mm in size. About 100 PLBs were obtained from 1.5 x 10 protoplasts. These PLB when transplanted onto Kyoto medium developed into plantlets which were transplanted and successfully grown under greenhouse conditions.

Additional Metadata

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Item Type: Proceedings Paper
Additional Information: Summary En
AGROVOC Term: Phalaenopsis
AGROVOC Term: Protoplasts
AGROVOC Term: Plantlets seedlings
AGROVOC Term: Callus
AGROVOC Term: Callus culture
Geographical Term: MALAYSIA
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:28
URI: http://webagris.upm.edu.my/id/eprint/16636

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