Citation
Ichihashi S., . and Shigemura S., . (2005) Phalaenopsis and protoplast culture. [Proceedings Paper]
Abstract
Plantlets were obtained by culture of orchid protoplasts. Callus of Phalaenopsis Wedding March was pre-cultured on sucrose-free medium for 3 weeks. Protoplasts were isolated from the pre-cultured callus. About 10 of the protoplasts cultured in sorbitol or maltose containing media divided within 4 weeks. Eleven months were required for Phalaenopsis protoplasts to produce PLB about 2-3 mm in size. About 100 PLBs were obtained from 1.5 x 10 protoplasts. These PLB when transplanted onto Kyoto medium developed into plantlets which were transplanted and successfully grown under greenhouse conditions.
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Abstract
Plantlets were obtained by culture of orchid protoplasts. Callus of Phalaenopsis Wedding March was pre-cultured on sucrose-free medium for 3 weeks. Protoplasts were isolated from the pre-cultured callus. About 10 of the protoplasts cultured in sorbitol or maltose containing media divided within 4 weeks. Eleven months were required for Phalaenopsis protoplasts to produce PLB about 2-3 mm in size. About 100 PLBs were obtained from 1.5 x 10 protoplasts. These PLB when transplanted onto Kyoto medium developed into plantlets which were transplanted and successfully grown under greenhouse conditions.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Summary En |
| AGROVOC Term: | Phalaenopsis |
| AGROVOC Term: | Protoplasts |
| AGROVOC Term: | Plantlets seedlings |
| AGROVOC Term: | Callus |
| AGROVOC Term: | Callus culture |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/16636 |
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