Green capsule culture of Coelogyne species

Narmatha Bai V., . and Ananthan R., . and Jeyakodi L., . and Baskar C., . and Jayakalaimathy K., . and Aravithan K.M., . (2005) Green capsule culture of Coelogyne species. [Proceedings Paper]

The beauty and long life span of orchid flowers have made them an important component of commercial cut-flower exports in several countries. However the commercial importance has put the natural diversity of orchids at stake. The technique of green capsule culture has added a new dimension to conservation and commercialization of orchids. Coelogyne comprises about 140 species distributed from India throughout South East Asia China the Phillipines and islands of New Guinea. Although most species are easy to grow and produce long-lasting fragrant flowers they have not become popular with growers or received much attention from orchidists. The present study was attempted with a view to developing a protocol for mass propagation and conservation of four species of Coelogyne: C.mossiae vulnerable C. nervosa endemic C. breviscapa and C.flaccida. Among the three media tried C. mossiae germination was 100 in all the media but the development of protocorn was better on the MS medium. MS medium also proved to be most suitable for C. breviscapa and C. nervosa. KC medium was best suited for seed germination and development of protocorns of C. flaccida. For further seedling development the best medium was selected and supplemented with hormones and growth additives. NAA was found to enhance seedling growth on C. mossiae C. nervosa and C. flaccida whereas C. breviscapa seedling development was better in kinetin. Activated charcoal enhanced the growth of C. mossiae and C. flaccida. Peptone and yeast extract proved to be beneficial for seedling development of C. breviscapa and C. nervosa respectively. Seedlings were well established in potting mixture containing brick charcoal pieces sphagnum moss and vermiculite in a ratio 1:1:1:1.

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Abstract

The beauty and long life span of orchid flowers have made them an important component of commercial cut-flower exports in several countries. However the commercial importance has put the natural diversity of orchids at stake. The technique of green capsule culture has added a new dimension to conservation and commercialization of orchids. Coelogyne comprises about 140 species distributed from India throughout South East Asia China the Phillipines and islands of New Guinea. Although most species are easy to grow and produce long-lasting fragrant flowers they have not become popular with growers or received much attention from orchidists. The present study was attempted with a view to developing a protocol for mass propagation and conservation of four species of Coelogyne: C.mossiae vulnerable C. nervosa endemic C. breviscapa and C.flaccida. Among the three media tried C. mossiae germination was 100 in all the media but the development of protocorn was better on the MS medium. MS medium also proved to be most suitable for C. breviscapa and C. nervosa. KC medium was best suited for seed germination and development of protocorns of C. flaccida. For further seedling development the best medium was selected and supplemented with hormones and growth additives. NAA was found to enhance seedling growth on C. mossiae C. nervosa and C. flaccida whereas C. breviscapa seedling development was better in kinetin. Activated charcoal enhanced the growth of C. mossiae and C. flaccida. Peptone and yeast extract proved to be beneficial for seedling development of C. breviscapa and C. nervosa respectively. Seedlings were well established in potting mixture containing brick charcoal pieces sphagnum moss and vermiculite in a ratio 1:1:1:1.

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Item Type:	Proceedings Paper
Additional Information:	Summary En
AGROVOC Term:	Orchidaceae
AGROVOC Term:	Plant propagation
AGROVOC Term:	Seed germination
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:28
URI:	http://webagris.upm.edu.my/id/eprint/16644

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