Agrobacterium mediated transformation of orchid plants

Niimi Y., . and Li Chen, . and Hatano T., . (2005) Agrobacterium mediated transformation of orchid plants. [Proceedings Paper]

Agrobacterium-mediated transformation of embryogenic callus ECs of Neofinetia falcata Hu rhizomes of Cymbidium niveo-marginatum Makino shoot primodials SPs of Schomburgkia sanderiana and protocorms of Zygopetalum mackayi and protocorm-like bodies PLBs of Phalaenopsis sp. was examined in this research. Transformation by using microprojectile bombardment has been successfully applied to Dendrobium PLBs and Phalaenopsis PLBs. In contrast the other transformation methods for higher plants using Agrobacterium are not generally useful for monocotyledonous plants including orchids. In this research we tried to transform various cultures of orchids such as ECs rhizomes SPs protocorms and PLBs by using Agrobacterium tumefaciens. Transgenic plants in Orchidaceae mediated by A. tumefaciens strains LBA4404 and EHA101 are described here. Blue spots of GUS expression and the GUS gene were comfirmed with the GUS assay and PCR analysis in ECs of N. falcata rhizomes of Cym. niveo-marginatum SPs of S. sanderiana protocorms of Z. mackayi and PLBs of Phalaenopsis sp. High efficiency of Agrobacterium-mediated transformation was observed in rhizomes of Cym. niveo-marginatum by A. tumefaciens strain LBA4404. However blue spots were not observed in protocorms of Z. mackayi and PLBs of Phalaenopsis sp. during the course of the present investigation. Another A. tumefaciens strain EHA101 was effective whwn used protocorms of Z. mackayi and PLBs of Phalaenopsis sp. with the same reporter gene GUS.

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Abstract

Agrobacterium-mediated transformation of embryogenic callus ECs of Neofinetia falcata Hu rhizomes of Cymbidium niveo-marginatum Makino shoot primodials SPs of Schomburgkia sanderiana and protocorms of Zygopetalum mackayi and protocorm-like bodies PLBs of Phalaenopsis sp. was examined in this research. Transformation by using microprojectile bombardment has been successfully applied to Dendrobium PLBs and Phalaenopsis PLBs. In contrast the other transformation methods for higher plants using Agrobacterium are not generally useful for monocotyledonous plants including orchids. In this research we tried to transform various cultures of orchids such as ECs rhizomes SPs protocorms and PLBs by using Agrobacterium tumefaciens. Transgenic plants in Orchidaceae mediated by A. tumefaciens strains LBA4404 and EHA101 are described here. Blue spots of GUS expression and the GUS gene were comfirmed with the GUS assay and PCR analysis in ECs of N. falcata rhizomes of Cym. niveo-marginatum SPs of S. sanderiana protocorms of Z. mackayi and PLBs of Phalaenopsis sp. High efficiency of Agrobacterium-mediated transformation was observed in rhizomes of Cym. niveo-marginatum by A. tumefaciens strain LBA4404. However blue spots were not observed in protocorms of Z. mackayi and PLBs of Phalaenopsis sp. during the course of the present investigation. Another A. tumefaciens strain EHA101 was effective whwn used protocorms of Z. mackayi and PLBs of Phalaenopsis sp. with the same reporter gene GUS.

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Item Type:	Proceedings Paper
Additional Information:	Summary En
AGROVOC Term:	Agrobacterium
AGROVOC Term:	Orchidaceae
AGROVOC Term:	Genetic transformation
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:28
URI:	http://webagris.upm.edu.my/id/eprint/16652

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