Antimicrobial activities and mechanisms of truncated and amino-acid-substituted peptides derived from bacteriocin PZJ5

Zhou, T. and Huang, Y. Y. and Ling, X. Y. and Zhang, L. and Gu, Q. and Song, D. F. (2023) Antimicrobial activities and mechanisms of truncated and amino-acid-substituted peptides derived from bacteriocin PZJ5. International Food Research Journal (Malaysia), 30. pp. 1582-1592. ISSN 2231 7546

Truncation and amino acid residue substitution are common methods to optimise the design of antimicrobial peptides (AMPs). In the present work, seven truncated and residuesubstituted derivatives of Plantaricin ZJ5 (PZJ5) were designed and synthesised. PZJ5-5 was a truncation that simultaneously contained three substituted amino-acid residues, with enhanced antimicrobial activity and low haemolytic activity. The effects of PZJ5-5 on Escherichia coli microstructure were investigated using scanning and transmission electron microscopy, which indicated that its antibacterial mechanism was similar to PZJ5. C-terminal amidation of PZJ5-5 (PZJ5-7) was deleterious, and resulted in a dramatic reduction in potency against E. coli and Listeria monocytogenes, with no potency against the other three indicator bacteria. Truncation and residue substitution of bacteriocin PZJ5 changed its antimicrobial activities and specificities, which provided a rationale for bacteriocin design.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/30%20(06)%202023/20%20-...

Abstract

Truncation and amino acid residue substitution are common methods to optimise the design of antimicrobial peptides (AMPs). In the present work, seven truncated and residuesubstituted derivatives of Plantaricin ZJ5 (PZJ5) were designed and synthesised. PZJ5-5 was a truncation that simultaneously contained three substituted amino-acid residues, with enhanced antimicrobial activity and low haemolytic activity. The effects of PZJ5-5 on Escherichia coli microstructure were investigated using scanning and transmission electron microscopy, which indicated that its antibacterial mechanism was similar to PZJ5. C-terminal amidation of PZJ5-5 (PZJ5-7) was deleterious, and resulted in a dramatic reduction in potency against E. coli and Listeria monocytogenes, with no potency against the other three indicator bacteria. Truncation and residue substitution of bacteriocin PZJ5 changed its antimicrobial activities and specificities, which provided a rationale for bacteriocin design.

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Item Type:	Article
AGROVOC Term:	bacteriocins
AGROVOC Term:	peptides
AGROVOC Term:	antimicrobial properties
AGROVOC Term:	Escherichia coli
AGROVOC Term:	experimentation
AGROVOC Term:	electron microscopy
AGROVOC Term:	amino acids
AGROVOC Term:	food technology
AGROVOC Term:	food preservation
Geographical Term:	China
Uncontrolled Keywords:	truncation, bacteriocin, antimicrobial peptide, antimicrobial activity, C-terminal amidation, amino acid residue substitution
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	21 Mar 2025 06:24
Last Modified:	21 Mar 2025 06:24
URI:	http://webagris.upm.edu.my/id/eprint/1671

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