Effect of vitrification pretreatment and exposure to liquid nitrogen on the structural changes of rambutan Nephelium Lappaceum L. embryonic axis

Chua C.K., . and U.R. Sinniah, . and A.G. Yunus, . and A.R Razak, . (2003) Effect of vitrification pretreatment and exposure to liquid nitrogen on the structural changes of rambutan Nephelium Lappaceum L. embryonic axis. [Proceedings Paper]

Germplasm conservation of species producing recalcitrant seeds is a problem and poses a great challenge. Despite cryopreservation being mentioned as the only possible method for conservation of such materials success has been limited and the sporadic survival is often not reproducible. The approach to cryopreservation is usually through empiric means by screening for tolerance to various cryoprotectant cocktails and testing for the best exposure duration. This results in little understanding of the effect of the substance used to the embryos. This study details the effect of exposure to vitrification cocktail prior and subsequent to liquid nitrogen exposure on the structural changes in rambutan zygotic embryonic axes as compared to the structure of freshly excised embryonic axes. Severe damage to cell structure was observed when freshly excised embryonic axes were cryopreserved without pretreatment. The pretreatment with vitrification cocktail helped to reduce damages to overall cell or tissue structure of the embryonic axis after cryopreservation.

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Abstract

Germplasm conservation of species producing recalcitrant seeds is a problem and poses a great challenge. Despite cryopreservation being mentioned as the only possible method for conservation of such materials success has been limited and the sporadic survival is often not reproducible. The approach to cryopreservation is usually through empiric means by screening for tolerance to various cryoprotectant cocktails and testing for the best exposure duration. This results in little understanding of the effect of the substance used to the embryos. This study details the effect of exposure to vitrification cocktail prior and subsequent to liquid nitrogen exposure on the structural changes in rambutan zygotic embryonic axes as compared to the structure of freshly excised embryonic axes. Severe damage to cell structure was observed when freshly excised embryonic axes were cryopreserved without pretreatment. The pretreatment with vitrification cocktail helped to reduce damages to overall cell or tissue structure of the embryonic axis after cryopreservation.

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Item Type:	Proceedings Paper
Additional Information:	Summary En
AGROVOC Term:	RAMBUTANS
AGROVOC Term:	NEPHELIUM LAPPACEUM
AGROVOC Term:	VITRIFICATION
AGROVOC Term:	LIQUID NITROGEN
AGROVOC Term:	STRUCTURAL CHANGE
AGROVOC Term:	FREEZING
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:28
URI:	http://webagris.upm.edu.my/id/eprint/16905

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