Citation
Lam P. F., . and Azlina S., . (2003) Rapid PCR screening of transgenic pineapple using FTA cards. [Proceedings Paper]
Abstract
A method for the screening of transgenic pineapple plants is described. DNA from the leaves was transferred onto FTA card. A disc of 2 mm diameter was punched from the FTA card and put into a PCR tube. The FTA card was washed twice with FTA purification buffer and twice with TE buffe dried and a PCR mix added. The primers used screened for part of the promoter of the antisense gene and the npt II genes. This method is rapid and does not make use of chemicals to extract DNA. Moreover the DNA in the FTA card be easily stored at room temperature.
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Abstract
A method for the screening of transgenic pineapple plants is described. DNA from the leaves was transferred onto FTA card. A disc of 2 mm diameter was punched from the FTA card and put into a PCR tube. The FTA card was washed twice with FTA purification buffer and twice with TE buffe dried and a PCR mix added. The primers used screened for part of the promoter of the antisense gene and the npt II genes. This method is rapid and does not make use of chemicals to extract DNA. Moreover the DNA in the FTA card be easily stored at room temperature.
Additional Metadata
| Item Type: | Proceedings Paper |
|---|---|
| Additional Information: | Received on 3 Dec 2007 QK710.2 M239 2003 |
| AGROVOC Term: | TRANSGENIC PLANTS |
| AGROVOC Term: | PINEAPPLES |
| AGROVOC Term: | TESTING |
| AGROVOC Term: | IMMOBILIZATION |
| AGROVOC Term: | GENES |
| Geographical Term: | MALAYSIA |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:28 |
| URI: | http://webagris.upm.edu.my/id/eprint/16939 |
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