Determination of antioxidant activities in various organic extracts from different parts of local Citrus grandis limau bali

Mashitoh A.R., . and Radzali M., . and Mahmud T.M.M., . (2003) Determination of antioxidant activities in various organic extracts from different parts of local Citrus grandis limau bali. [Proceedings Paper]

This preliminary study was analytically performed in order to investigate the percentage of yields and antioxidant activities of various organic extracts such as diethylether ethanol methanol and water from peels leaves and juices of Citrus grandis. Among four organic solvents used ethanol extract of peels yielded of:29.733 - 0.64 juices: 26.05 - 0.97 and leaves: 17.83 - 0.5 and methanol extract of peel yields: 20.153 - 0.45 juices: 25.86 - 0.75 and leaves: 19.37 - 0.80 are showed the most effective in extracting of studied of C. grandis respectively compared to diethylether extract of peels yielded of : 2.60 - 0.11 juices: 14.27 - 0.87 and leaves 10.132 - 0.42. However water extract also showed an appreciable yield. Results indicated that the yield of most extract was greater with the more polar solvents used. Antioxidant activities of various extracts were biochemically evaluated by using two established tests: - a Linoleic acid model system conjugated diene and b 1 1-diphenyl-2-picrylhydrazyl DPPH scavenging test. The antioxidant activities were scientifically compared with authentic standard antioxidants such as butylated hydroxytoluene BHT and alpha-tocopherol Vitamin E. Results of conjugated diene test were as followed for ethanolic extract of peels: 0.97 - 0.03 activity per 10 microsoluble extracts leaves: 0.775 - 0.006 activity per 10 micro soluble extracts and juices: 0.74 - 0.06 activity per 10 micro soluble extracts methanolic extract of peel: 0.818 - 0.02 activity per 10 micro soluble extracts leaves: 0.884 - 0.02 activity per 10 micro soluble extract juices: 0.70 - 0.03 activity per 10 micro soluble extract water extract of peel: 0.924 - 0.03 activity per 10 micro soluble extracts leaves: 0.653 - 0.02 activity per 10 micro soluble extracts and juices 0.615 - 0.04 activity per 10 micro soluble extracts and diethylether extract of peels: 0.15 - 0.04 activity per 10 micro soluble extracts leaves: 0.102 - 03 activity per 10 micro soluble extracts. Results also exhibited that antioxidant activity of peel in ethanolic extract 0.97 - 0.03 activity per 10 micro soluble extracts was found to be a best as compared to synthethic antioxidant butylated hydroxytoluene BHT 0.98 - 0.04 activity per 10 micro soluble extracts. DPPH antioxidant activity test showed a positive result by decreasing absorbance with time in all types of organic solvents used for different samples of Citrus grandis.

Full text available from:

Abstract

This preliminary study was analytically performed in order to investigate the percentage of yields and antioxidant activities of various organic extracts such as diethylether ethanol methanol and water from peels leaves and juices of Citrus grandis. Among four organic solvents used ethanol extract of peels yielded of:29.733 - 0.64 juices: 26.05 - 0.97 and leaves: 17.83 - 0.5 and methanol extract of peel yields: 20.153 - 0.45 juices: 25.86 - 0.75 and leaves: 19.37 - 0.80 are showed the most effective in extracting of studied of C. grandis respectively compared to diethylether extract of peels yielded of : 2.60 - 0.11 juices: 14.27 - 0.87 and leaves 10.132 - 0.42. However water extract also showed an appreciable yield. Results indicated that the yield of most extract was greater with the more polar solvents used. Antioxidant activities of various extracts were biochemically evaluated by using two established tests: - a Linoleic acid model system conjugated diene and b 1 1-diphenyl-2-picrylhydrazyl DPPH scavenging test. The antioxidant activities were scientifically compared with authentic standard antioxidants such as butylated hydroxytoluene BHT and alpha-tocopherol Vitamin E. Results of conjugated diene test were as followed for ethanolic extract of peels: 0.97 - 0.03 activity per 10 microsoluble extracts leaves: 0.775 - 0.006 activity per 10 micro soluble extracts and juices: 0.74 - 0.06 activity per 10 micro soluble extracts methanolic extract of peel: 0.818 - 0.02 activity per 10 micro soluble extracts leaves: 0.884 - 0.02 activity per 10 micro soluble extract juices: 0.70 - 0.03 activity per 10 micro soluble extract water extract of peel: 0.924 - 0.03 activity per 10 micro soluble extracts leaves: 0.653 - 0.02 activity per 10 micro soluble extracts and juices 0.615 - 0.04 activity per 10 micro soluble extracts and diethylether extract of peels: 0.15 - 0.04 activity per 10 micro soluble extracts leaves: 0.102 - 03 activity per 10 micro soluble extracts. Results also exhibited that antioxidant activity of peel in ethanolic extract 0.97 - 0.03 activity per 10 micro soluble extracts was found to be a best as compared to synthethic antioxidant butylated hydroxytoluene BHT 0.98 - 0.04 activity per 10 micro soluble extracts. DPPH antioxidant activity test showed a positive result by decreasing absorbance with time in all types of organic solvents used for different samples of Citrus grandis.

[error in script]

Item Type:	Proceedings Paper
Additional Information:	Received on 3 Dec 2007 QK710.2 M239 2003
AGROVOC Term:	CITRUS GRANDIS
AGROVOC Term:	ANTIOXIDANTS
AGROVOC Term:	ORGANIC MATTER
AGROVOC Term:	PUMMELOS
AGROVOC Term:	TISSUE ANALYSIS
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:28
URI:	http://webagris.upm.edu.my/id/eprint/16940

Actions (login required)

View Item