Construction of a plant expression vector for suppression subtractive hybridization cDNA library application

Tan C.S., . and Norliza A.B., . (2003) Construction of a plant expression vector for suppression subtractive hybridization cDNA library application. [Proceedings Paper]

To accelerate gene isolation from plant and in order to carry out functional genomics studies of plant genes we must be able to readily manipulate and express all the genes in a vector driven by a promoter that is suitable for plant gene study expression. Most of the available vectors in the market are utilizing bacterial promoter and thus not suitable for plant gene study. Two vectors were designed to allow directional cloning and expression of plant cDNAs that have undergone the Suppression Subtractive Hybridization procedure. The plasmid and phage library constructed are driven by CaMV35S a constitutive promoter that allows the expression of naked plant cDNAs that will be introduced into plant cells via particle bombardment.

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Abstract

To accelerate gene isolation from plant and in order to carry out functional genomics studies of plant genes we must be able to readily manipulate and express all the genes in a vector driven by a promoter that is suitable for plant gene study expression. Most of the available vectors in the market are utilizing bacterial promoter and thus not suitable for plant gene study. Two vectors were designed to allow directional cloning and expression of plant cDNAs that have undergone the Suppression Subtractive Hybridization procedure. The plasmid and phage library constructed are driven by CaMV35S a constitutive promoter that allows the expression of naked plant cDNAs that will be introduced into plant cells via particle bombardment.

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Item Type:	Proceedings Paper
Additional Information:	Received on 3 Dec 2007 QK710.2 M239 2003
AGROVOC Term:	VECTORS
AGROVOC Term:	PLANTS
AGROVOC Term:	PLASMIDS
AGROVOC Term:	CLONES
AGROVOC Term:	HYBRIDIZATION
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:28
URI:	http://webagris.upm.edu.my/id/eprint/16941

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