A fruit specific and ripening related endopolygalacturonase gene from papaya Carica papaya L.

Roohaida O., . and Tan W.F., . and Zainon M. A., . and Hamid L., . (2003) A fruit specific and ripening related endopolygalacturonase gene from papaya Carica papaya L. [Proceedings Paper]

Fruit ripening is caused by a series of cordinated physiological and biochemical processes resulting in changes in its texture. Fruit softening is associated with extensive solubilization and degradation of cell-wall polysaccharides which result from the action of a number of cell-wall hydrolytic enzymes including polygalacturonase. Endopolygalacturonase has been extensively studied in tomato fruit where it accumulates during ripening and is responsible for the degradation of polyuronides in the fruit cell wall. A fruit specific and ripening related endopolygalacturonse clone 2.5kb in length has been isolated from papaya cDNA library using differential screening method. The papaya endopolygalacturonse transcript showed increasing expression during fruit ripening but was undetectable in other tissues hence indicating its specificity in repening fruit tissues.

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Abstract

Fruit ripening is caused by a series of cordinated physiological and biochemical processes resulting in changes in its texture. Fruit softening is associated with extensive solubilization and degradation of cell-wall polysaccharides which result from the action of a number of cell-wall hydrolytic enzymes including polygalacturonase. Endopolygalacturonase has been extensively studied in tomato fruit where it accumulates during ripening and is responsible for the degradation of polyuronides in the fruit cell wall. A fruit specific and ripening related endopolygalacturonse clone 2.5kb in length has been isolated from papaya cDNA library using differential screening method. The papaya endopolygalacturonse transcript showed increasing expression during fruit ripening but was undetectable in other tissues hence indicating its specificity in repening fruit tissues.

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Item Type:	Proceedings Paper
Additional Information:	Received on 3 Dec 2007 QK710.2 M239 2003
AGROVOC Term:	PAPAYAS
AGROVOC Term:	RIPENING
AGROVOC Term:	ISOLATION
AGROVOC Term:	TISSUE ANALYSIS
AGROVOC Term:	CLONES
AGROVOC Term:	CARICA PAPAYA
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:28
URI:	http://webagris.upm.edu.my/id/eprint/16943

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