Mutation induction and propagation of Asplenium nidus a fern species from spores

Norazlina N., . and Azhar M., . and Rusli I., . (2003) Mutation induction and propagation of Asplenium nidus a fern species from spores. [Proceedings Paper]

Ferns are one of the most diverse group of living land plants it is estimated that there are 11 000 species in 300 genera. One of the well-known species ia Aspleniumnidus. This fern is also well-known as Birds-nest fern a medium to large fern with erect stout unbranched rhizomes. In creating variability for ferns mutation induction is used as an alternative way to change the characteristics. Information on the radiosensitivity test is an important factor in deciding the approach to be taken for genetic changes of the fern in mutation breeding. In this study spore cultures os Asplenium nidus were irradiated with doses of 0 10 20 30 90 and 180 Gy using gamma cell with 60Co source at dose rate of 0.25Gy s-1. It was found that the most suitable dose for spore culture induction was at 20 Gy. Both irradiated spore cultures 20 Gy and control cultures 0 Gy were then cultured onto semi-solid modified 1/2 MS basal medium without hormone. Spore cultures were incubated in growth room at 24 deg C with 16 hours photoperiod 3500 lux. After 1-2 months of culture it was observed that young gametophytes green in colour production was higher for irradiated spore cultures 20 Gy when compared to the control cultures 0 Gy. The ptoduction was about 68 for Gy spore cultures and at 20 for 0 Gy. Gametophytes were subcultured at monthly interval to ensure futher development and propagation. Later plantlets were then transferred to nursery for hardening process for about 1-2 months and will be then maintained for futher propagation and frequently monitored for any changes in the morphology of the irradiated Asplenium nidus plants.

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Abstract

Ferns are one of the most diverse group of living land plants it is estimated that there are 11 000 species in 300 genera. One of the well-known species ia Aspleniumnidus. This fern is also well-known as Birds-nest fern a medium to large fern with erect stout unbranched rhizomes. In creating variability for ferns mutation induction is used as an alternative way to change the characteristics. Information on the radiosensitivity test is an important factor in deciding the approach to be taken for genetic changes of the fern in mutation breeding. In this study spore cultures os Asplenium nidus were irradiated with doses of 0 10 20 30 90 and 180 Gy using gamma cell with 60Co source at dose rate of 0.25Gy s-1. It was found that the most suitable dose for spore culture induction was at 20 Gy. Both irradiated spore cultures 20 Gy and control cultures 0 Gy were then cultured onto semi-solid modified 1/2 MS basal medium without hormone. Spore cultures were incubated in growth room at 24 deg C with 16 hours photoperiod 3500 lux. After 1-2 months of culture it was observed that young gametophytes green in colour production was higher for irradiated spore cultures 20 Gy when compared to the control cultures 0 Gy. The ptoduction was about 68 for Gy spore cultures and at 20 for 0 Gy. Gametophytes were subcultured at monthly interval to ensure futher development and propagation. Later plantlets were then transferred to nursery for hardening process for about 1-2 months and will be then maintained for futher propagation and frequently monitored for any changes in the morphology of the irradiated Asplenium nidus plants.

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Item Type:	Proceedings Paper
Additional Information:	Received on 3 Dec 2007 QK710.2 M239 2003
AGROVOC Term:	STERILIZING
AGROVOC Term:	FERNS
AGROVOC Term:	SPORES
AGROVOC Term:	MUTATION
AGROVOC Term:	IRRADIATION
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:28
URI:	http://webagris.upm.edu.my/id/eprint/16948

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