Somatic embryo formation and germination from male flower cluster-derived suspensions of banana cultivar rastali

Azlin A. M. Z., . and Rashid A. A., . and Aziz M. A., . and Saleh N. M., . (2003) Somatic embryo formation and germination from male flower cluster-derived suspensions of banana cultivar rastali. [Proceedings Paper]

A protocol for somatic embryo formation and germination from male flower cluster-derived cell suspensions of banana cultivar Rastali was developed. Flower clusters from position one to ten were excised from male inflorescences and cultured onto modified M1 semi-solid medium. Callus were initiated from flower cluster positions five to ten within six months of culture Callus produced from the most responsive cluster positions were transferred onto Dhed a liquid medium to produce cell suspension cultures. The cell suspensions were sieved and subcultured thrice at an interval of four weeks. After the third subculture fine granules embryogenic cell clusters produced in the cell suspensions were transferred onto hormone-free solid MS maturation medium. Within two months the embryogenic cell clusters developed into embryogenic callus and then into whitish globular structures. The globular-stage somatic embryos were transferred onto germination medium and regenerated into plantlets.

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Abstract

A protocol for somatic embryo formation and germination from male flower cluster-derived cell suspensions of banana cultivar Rastali was developed. Flower clusters from position one to ten were excised from male inflorescences and cultured onto modified M1 semi-solid medium. Callus were initiated from flower cluster positions five to ten within six months of culture Callus produced from the most responsive cluster positions were transferred onto Dhed a liquid medium to produce cell suspension cultures. The cell suspensions were sieved and subcultured thrice at an interval of four weeks. After the third subculture fine granules embryogenic cell clusters produced in the cell suspensions were transferred onto hormone-free solid MS maturation medium. Within two months the embryogenic cell clusters developed into embryogenic callus and then into whitish globular structures. The globular-stage somatic embryos were transferred onto germination medium and regenerated into plantlets.

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Item Type:	Proceedings Paper
Additional Information:	Received on 3 Dec 2007 QK710.2 M239 2003
AGROVOC Term:	SOMATIC EMBRYOS
AGROVOC Term:	GERMINATION
AGROVOC Term:	FLOWERS
AGROVOC Term:	BANANAS
AGROVOC Term:	MUSA BANANAS
Geographical Term:	MALAYSIA
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:28
URI:	http://webagris.upm.edu.my/id/eprint/16982

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