The influence of fermentor operation on citric acid production by Aspergillus niger under nitrogen limitation


Citation

Arbakariya Ariff, . and Ismail Abdul Karim, . and Madihah Md Salleh, . The influence of fermentor operation on citric acid production by Aspergillus niger under nitrogen limitation. pp. 66-69. ISSN 1505-5337

Abstract

Production of citric acid has been studied using Aspergillus niger in batch continous and exponentially fed-batch fermentations. The fermentation were carried out using a one L stirred tank fermentor. In continous fermentation optimum citric acid production was obtained at a dilution rate of 0.07/h and a culture pH of 2.5. Fermentor productivity for batch continous and fed-batch fermentations were 0.025g/L.h 0.294 g/L.h and 0.061 g/L.h respectively. Although very high fermentor productivity was obtained in continous fermentation the steady state citric acid concentration in the outflow (4.2 g/L) was about one-third the maximum concentration of citric acid obtained in exponentially fed-batch culture (12.1 g/L). Yields based on nitrogen consumed for batch continous and fed-batch fermentation were 3.0 1.4 and 4.8 g citric acid/g ammonium sulphate respectively. In comparison with batch fermentation exponentially fed-batch fermentation improved citric acid production significantly in terms of yield and productivity.


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Abstract

Production of citric acid has been studied using Aspergillus niger in batch continous and exponentially fed-batch fermentations. The fermentation were carried out using a one L stirred tank fermentor. In continous fermentation optimum citric acid production was obtained at a dilution rate of 0.07/h and a culture pH of 2.5. Fermentor productivity for batch continous and fed-batch fermentations were 0.025g/L.h 0.294 g/L.h and 0.061 g/L.h respectively. Although very high fermentor productivity was obtained in continous fermentation the steady state citric acid concentration in the outflow (4.2 g/L) was about one-third the maximum concentration of citric acid obtained in exponentially fed-batch culture (12.1 g/L). Yields based on nitrogen consumed for batch continous and fed-batch fermentation were 3.0 1.4 and 4.8 g citric acid/g ammonium sulphate respectively. In comparison with batch fermentation exponentially fed-batch fermentation improved citric acid production significantly in terms of yield and productivity.

Additional Metadata

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Item Type: Article
Additional Information: Summary (En)
AGROVOC Term: FERMENTACION
AGROVOC Term: TECNICAS DE CULTIVO
AGROVOC Term: ASPERGILLUS NIGER
AGROVOC Term: INDICE DE CRECIMIENTO
AGROVOC Term: ACIDO CITRICO
AGROVOC Term: FERMENTADORES
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:51
URI: http://webagris.upm.edu.my/id/eprint/17028

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