Identification and cross-breeding of gamma irradiation-induced auxotrophs of Agaricus bitorquis


Citation

Teow S.S., . and Zakri Abdul Hamid, . and Promwichit P., . and Graham K.M., . Identification and cross-breeding of gamma irradiation-induced auxotrophs of Agaricus bitorquis. pp. 59-65. ISSN 0126-8643

Abstract

Thirty eight auxotrophs of Agaricus bitorquis were isolated and identified from a total of 10 000 gamma irradiated monosporous isolates comprising of five different stocks. The highest incidence rate of auxotrophs was recovered at 0.45 percent survival and the autotrophic mutants exhibited different nutritional requirements for growth on minimal medium. Sixty eight pairings were positive for dikaryon formation out of 128 intrastock matings and a total of 505 matings were positive out of 575 interstock crosses. Compatible matings between homokaryotic isolates resulted in the development of district fertile dikaryons which could be easily identified and selected. Two of the hybrids were better producers yielding additions of 18 and 22 percent more of sporocarps as compared to their wild parental stocks signifying that mutagenesis followed by cross-breeding could serve as an effective means in strain improvement of edible mushroom through the hybridization of genes of agronomic importance


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Abstract

Thirty eight auxotrophs of Agaricus bitorquis were isolated and identified from a total of 10 000 gamma irradiated monosporous isolates comprising of five different stocks. The highest incidence rate of auxotrophs was recovered at 0.45 percent survival and the autotrophic mutants exhibited different nutritional requirements for growth on minimal medium. Sixty eight pairings were positive for dikaryon formation out of 128 intrastock matings and a total of 505 matings were positive out of 575 interstock crosses. Compatible matings between homokaryotic isolates resulted in the development of district fertile dikaryons which could be easily identified and selected. Two of the hybrids were better producers yielding additions of 18 and 22 percent more of sporocarps as compared to their wild parental stocks signifying that mutagenesis followed by cross-breeding could serve as an effective means in strain improvement of edible mushroom through the hybridization of genes of agronomic importance

Additional Metadata

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Item Type: Article
Additional Information: Summary (En)
AGROVOC Term: AGARICUS
AGROVOC Term: IDENTIFICACION
AGROVOC Term: HIBRIDACION
AGROVOC Term: IRRADIACION GAMMA
AGROVOC Term: AUXOTROFIA
AGROVOC Term: NECESIDADES DE NUTRIENTES
AGROVOC Term: HIBRIDOS
AGROVOC Term: MUTANTES
AGROVOC Term: SETAS COMESTIBLES
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:51
URI: http://webagris.upm.edu.my/id/eprint/17069

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