The influence of low temperature treatment on growth and proline accumulation in polyembryogenic cultures of oil palm (Elaeis guineensis Jacq.)


Citation

Tarmizi A.H., . The influence of low temperature treatment on growth and proline accumulation in polyembryogenic cultures of oil palm (Elaeis guineensis Jacq.). pp. 107-117. ISSN 0128-1828

Abstract

Low temperatures (15 degree C-20 degree C) were observed to inhibit the growth and multiplication of oil palm polyembryorgenic (PE) cultures. These conditions enable PE cultures to be kept for at least six months without subculturing. The method can therefore be exploited for minimal growth storage of in vitro cultures. Proline which is known to be a universal stress indicator was found to accumulate in the cultures under low temperature stress and the increase was about two-fold during 30 days of exposure. In the presence of 0.5 M sucrose low temperature storage of cultures could be extended even to nine months.


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Abstract

Low temperatures (15 degree C-20 degree C) were observed to inhibit the growth and multiplication of oil palm polyembryorgenic (PE) cultures. These conditions enable PE cultures to be kept for at least six months without subculturing. The method can therefore be exploited for minimal growth storage of in vitro cultures. Proline which is known to be a universal stress indicator was found to accumulate in the cultures under low temperature stress and the increase was about two-fold during 30 days of exposure. In the presence of 0.5 M sucrose low temperature storage of cultures could be extended even to nine months.

Additional Metadata

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Item Type: Article
Additional Information: ill.; 2 tables; 36 ref.
AGROVOC Term: PALMAS OLEAGINOSAS
AGROVOC Term: ELAEIS GUINEENSIS
AGROVOC Term: CULTIVO DE EMBRIONES
AGROVOC Term: CULTIVO IN VITRO
AGROVOC Term: CRECIMIENTO
AGROVOC Term: PROLINA
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:51
URI: http://webagris.upm.edu.my/id/eprint/17129

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