Citation
Sunderasan E., . and Samsidar Hamzah, . and Sharifah Hamid, . and Ward M.A., . and Yeang H.Y., . and Cardosa M.J., . Latex B-serum -1 3-Glucanase (Hev b II) and a component of the microhelix (Hev b IV) are major latex allergens. pp. 82-99. ISSN 0127-7065
Abstract
Two allergenic (IgE-binding) proteins have been isolated from the B-serum of natural rubber latex by dialysis-induced protein precipitation followed by gel filtration. One of the allergens Hev b II that is specific to the monoclonal antibody USM/RB4 commonly appears as a doublet of molecular weights 34 and 36 kDa under reducing conditions of SDS-polyacrylamide gel electrophoresis. In the absense of 2-mercaptoethanol a 70 kDa band can also be seen in addition to the doublet. The allergen has a pI of circa 9.5 and is identified as B-1 3-glucanase. The second allergen Hev b IV has a pI at circa 4.5 and appears under reducing conditions as a band of 50-57 kDa. In its unreduced form a principal band of approximately 100 kDa is discernible. Immuno-gold labelling with the monoclonal antibody USM/RB3 shows that Hev b IV is a component of the microhelix protein complex of B-serum.
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Abstract
Two allergenic (IgE-binding) proteins have been isolated from the B-serum of natural rubber latex by dialysis-induced protein precipitation followed by gel filtration. One of the allergens Hev b II that is specific to the monoclonal antibody USM/RB4 commonly appears as a doublet of molecular weights 34 and 36 kDa under reducing conditions of SDS-polyacrylamide gel electrophoresis. In the absense of 2-mercaptoethanol a 70 kDa band can also be seen in addition to the doublet. The allergen has a pI of circa 9.5 and is identified as B-1 3-glucanase. The second allergen Hev b IV has a pI at circa 4.5 and appears under reducing conditions as a band of 50-57 kDa. In its unreduced form a principal band of approximately 100 kDa is discernible. Immuno-gold labelling with the monoclonal antibody USM/RB3 shows that Hev b IV is a component of the microhelix protein complex of B-serum.
Additional Metadata
| Item Type: | Article |
|---|---|
| Additional Information: | Summary (En) |
| AGROVOC Term: | CAUCHO |
| AGROVOC Term: | LATEX |
| AGROVOC Term: | ALERGENOS |
| AGROVOC Term: | PROTEINAS |
| AGROVOC Term: | DIALISIS |
| AGROVOC Term: | FILTRACION |
| AGROVOC Term: | REACCIONES ALERGICAS |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:51 |
| URI: | http://webagris.upm.edu.my/id/eprint/17150 |
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