Citation
Aitchitt M., . and Mantell S.H., . and Thangavellu M., . and Ainsworth C.C., . Cloning date palm (Phoenix dactylifera L.) DNA and characterization of low medium and high copy DNA sequences. pp. 57-63. ISSN 0128-1828
Abstract
Total DNA was extracted from mature leaves of the date palm (Phoenix dactylifera L.) cultivar 'Bou-Sthammi Noire'. The DNA was digested with Pst1 and the fragments shotgun-cloned in E. coli DH5 using the plasmid vector pUC19. A total of 124 clones were characterised by dot blot hybridization using 32P labelled genomic DNA as the probe. 74 percent of the isolates contained single copy sequences. Many of the cloned fragments (58 percent) were less than 900 bp. Some of the single copy clones when used as probes in Southern blot analysis of DNA extracted from twelve Moroccan date palm cultivars detected restriction fragment length polymorphisms. The construction and the availability of a genomic library opens up the possibility of obtaining a panel of potentially interesting genomic DNA clones for initiating RFLP studies on breeding line of Moroccan date palms in order to speed up the selection and breeding of Bayoud resistant palms in Morocco
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Abstract
Total DNA was extracted from mature leaves of the date palm (Phoenix dactylifera L.) cultivar 'Bou-Sthammi Noire'. The DNA was digested with Pst1 and the fragments shotgun-cloned in E. coli DH5 using the plasmid vector pUC19. A total of 124 clones were characterised by dot blot hybridization using 32P labelled genomic DNA as the probe. 74 percent of the isolates contained single copy sequences. Many of the cloned fragments (58 percent) were less than 900 bp. Some of the single copy clones when used as probes in Southern blot analysis of DNA extracted from twelve Moroccan date palm cultivars detected restriction fragment length polymorphisms. The construction and the availability of a genomic library opens up the possibility of obtaining a panel of potentially interesting genomic DNA clones for initiating RFLP studies on breeding line of Moroccan date palms in order to speed up the selection and breeding of Bayoud resistant palms in Morocco
Additional Metadata
| Item Type: | Article |
|---|---|
| Additional Information: | Summary (En) |
| AGROVOC Term: | PHOENIX DACTYLIFERA |
| AGROVOC Term: | CLONACION MOLECULAR |
| AGROVOC Term: | RECOMBINACION |
| AGROVOC Term: | ADN RECOMBINADO |
| AGROVOC Term: | GENOMAS |
| AGROVOC Term: | DATIL |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:51 |
| URI: | http://webagris.upm.edu.my/id/eprint/17189 |
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