Citation
Zehr Bradley D., . and Kostyal David A., . and Beezhold Donald, . Stability of antigenic protein in latex extracts. pp. 15-18. ISSN 0035-9483
Abstract
Residual proteins on latex medical products are responsible for IgE mediated allergic reactions. Accurate methods for quantifying latex proteins are important for proper product quality control. An important parameter involved in quantifying latex proteins is understanding protein stability in the extraction medium. In this study we examined the stability of latex proteins in water and various buffered solutions using the latex ELISA for antigenic protein (LEAP). The data demonstrate short term stability (1-2 weeks) of latex proteins at room temperature in all solutions tested. Long term stability of latex proteins was found in carbonate buffer (pH 9.6) and acetate buffer (pH 5.5). This study provides additional information regarding the preparation and storage of purified latex protein reference solutions or control extracts.
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Abstract
Residual proteins on latex medical products are responsible for IgE mediated allergic reactions. Accurate methods for quantifying latex proteins are important for proper product quality control. An important parameter involved in quantifying latex proteins is understanding protein stability in the extraction medium. In this study we examined the stability of latex proteins in water and various buffered solutions using the latex ELISA for antigenic protein (LEAP). The data demonstrate short term stability (1-2 weeks) of latex proteins at room temperature in all solutions tested. Long term stability of latex proteins was found in carbonate buffer (pH 9.6) and acetate buffer (pH 5.5). This study provides additional information regarding the preparation and storage of purified latex protein reference solutions or control extracts.
Additional Metadata
| Item Type: | Article |
|---|---|
| Additional Information: | Summary (En) |
| AGROVOC Term: | LATEX |
| AGROVOC Term: | CAUCHO |
| AGROVOC Term: | TECNICAS ANALITICAS |
| AGROVOC Term: | PROTEINAS |
| AGROVOC Term: | RESIDUOS |
| AGROVOC Term: | PROTEINAS DE RESERVA |
| AGROVOC Term: | REACCIONES ALERGICAS |
| AGROVOC Term: | CONTROL DE CALIDAD |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:51 |
| URI: | http://webagris.upm.edu.my/id/eprint/17252 |
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