Recent advances in the development of molecular markers for Hevea studies

Low F.C., . and Safiah Atan, . and Hafsah Jaafar, . and Tan H., . Recent advances in the development of molecular markers for Hevea studies. pp. 32-44. ISSN 0127-7065

Four DNA molecular marker techniques were developed and applied to Hevea studies. These included the conventional Southern-based marker technique called restriction fragment length polymorphisms (RFLPs) and three polymerase chain reaction amplification-based techniques viz. random amplified polymorphic DNA microsatellites and DNA amplification fingerprinting (DAF). These molecular markers were able to elicit interspecific as well as intraspecific polymorphisms. There were also able to assist in several identification studies. Cultivars which share two common parents (siblings) such as PR 255 and PR 261; RRIM 901 and RRIM 905; and RRIM 937 and RRIM 938 were distinguished by their DNA polymorphisms. Similarly molecular markers had assisted the identification of 'rogues' in a sample of monoclonal PB 5/51 seedlings. The genetic diversity of genotypes from the 1981 IRRDB Hevea germplasm collection from brazil was displayed and confirmed by their RFLPs. For the first time microsatellites had been detected in the Hevea genome. Three microsatellite sequences were detected within one gene (hydroxylmethyl-glutaryl coenzyme A reductase-1). Preliminary evidence suggested that one of these microsatellite sequences (GA)9 was polymorphic. A reduction in the number of ribosomal DNA (rDNA) genes during cell culture was suggested when pTa71 a wheat rDNA probe was hybridised with digested gDNA obtained from in vitro culture plants. Somaclonal variations were detected among a population of GL 1 plants derived from another and ovule culture by a recently developed DNA fingerprinting technique called DAF. A genetic linkage map comprising of RFLPs and other markers is being constructed. The advantages and disadvantages of these marker technique were compared

Full text available from:

Abstract

Four DNA molecular marker techniques were developed and applied to Hevea studies. These included the conventional Southern-based marker technique called restriction fragment length polymorphisms (RFLPs) and three polymerase chain reaction amplification-based techniques viz. random amplified polymorphic DNA microsatellites and DNA amplification fingerprinting (DAF). These molecular markers were able to elicit interspecific as well as intraspecific polymorphisms. There were also able to assist in several identification studies. Cultivars which share two common parents (siblings) such as PR 255 and PR 261; RRIM 901 and RRIM 905; and RRIM 937 and RRIM 938 were distinguished by their DNA polymorphisms. Similarly molecular markers had assisted the identification of 'rogues' in a sample of monoclonal PB 5/51 seedlings. The genetic diversity of genotypes from the 1981 IRRDB Hevea germplasm collection from brazil was displayed and confirmed by their RFLPs. For the first time microsatellites had been detected in the Hevea genome. Three microsatellite sequences were detected within one gene (hydroxylmethyl-glutaryl coenzyme A reductase-1). Preliminary evidence suggested that one of these microsatellite sequences (GA)9 was polymorphic. A reduction in the number of ribosomal DNA (rDNA) genes during cell culture was suggested when pTa71 a wheat rDNA probe was hybridised with digested gDNA obtained from in vitro culture plants. Somaclonal variations were detected among a population of GL 1 plants derived from another and ovule culture by a recently developed DNA fingerprinting technique called DAF. A genetic linkage map comprising of RFLPs and other markers is being constructed. The advantages and disadvantages of these marker technique were compared

[error in script]

Item Type:	Article
Additional Information:	Summary (En)
AGROVOC Term:	HEVEA
AGROVOC Term:	FITOMEJORAMIENTO
AGROVOC Term:	GENETICA MOLECULAR
AGROVOC Term:	ADN
AGROVOC Term:	HIBRIDACION
AGROVOC Term:	CULTIVO IN VITRO
AGROVOC Term:	RFLP
AGROVOC Term:	MARCADORES GENETICOS
AGROVOC Term:	PCR
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:51
URI:	http://webagris.upm.edu.my/id/eprint/17294

Actions (login required)

View Item