The milk curdling ability of crude protease of sardine (Sardinops melanastictus) offal in the production of dadih.


Citation

Jamilah Bakar, . and Siti Salwana Hashim, . The milk curdling ability of crude protease of sardine (Sardinops melanastictus) offal in the production of dadih. pp. 95-98. ISSN 1505-5337

Abstract

A protease extract in dry acetone powder form was prepared from sardine (Sardinops melanastictus) offal. Its milk clotting activity was studied for the production of dadih. It has an optimum pH of 5.0 and an optimum temperature of 60 degree C. The protease was able to curdle milk in the absence if CaCl2. A minimun of 0.6 percent of the extract containing 11.22 mg/mL protein with a proteolytic activity of 54.43 u/min/mL on haemoglobin or 11.32 u/min/mL on casein was required for milk clotting activity.


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Abstract

A protease extract in dry acetone powder form was prepared from sardine (Sardinops melanastictus) offal. Its milk clotting activity was studied for the production of dadih. It has an optimum pH of 5.0 and an optimum temperature of 60 degree C. The protease was able to curdle milk in the absence if CaCl2. A minimun of 0.6 percent of the extract containing 11.22 mg/mL protein with a proteolytic activity of 54.43 u/min/mL on haemoglobin or 11.32 u/min/mL on casein was required for milk clotting activity.

Additional Metadata

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Item Type: Article
Additional Information: Summary (En)
AGROVOC Term: SARDINES
AGROVOC Term: SARDINOPS
AGROVOC Term: CRUDE PROTEIN
AGROVOC Term: COAGULATION
AGROVOC Term: MILK
AGROVOC Term: PROTEASES
AGROVOC Term: STREBLUS ASPER
AGROVOC Term: PLANT EXTRACTS
AGROVOC Term: CHYMOSIN
AGROVOC Term: MALAYSIA SARDINA
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:52
URI: http://webagris.upm.edu.my/id/eprint/17464

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