Citation
Roy A., . and Saha P.K., . Factors involved during in vitro culture of Calamus rotang. pp. 225-232. ISSN 0128-1283
Abstract
Roy A. Saha P.K. 1997. Factors involved during in vitro culture of Calamus rotang. Calamus rotang is a subterranean woody monocot where in vitro multiplication technique may be used as an alternative process for rapid plant generation. Since subterrestrial young plantlets face a problem in establishment due to a high degree of contamination in the medium to reduce contamination chemicals were tested for sterilisation of the explant. Sodium hypochlorite (5.0 percent v/v) in combination with mercuric chloride (0.5 percent w/v) resulted in the highest response. Explant establishment was dependent on their lengths as well as the plantlets from where the explants were derived. Explants of length 1.5-2.0 cm derived from plantlets having a length of 10-20 cm showed a higher rate of growth under culture conditions. Bud proliferation in explant was achieved in the establisment medium supplemented with BAP (6-benzylaminopurine) (5-10mg per l).
Download File
Full text available from:
|
Abstract
Roy A. Saha P.K. 1997. Factors involved during in vitro culture of Calamus rotang. Calamus rotang is a subterranean woody monocot where in vitro multiplication technique may be used as an alternative process for rapid plant generation. Since subterrestrial young plantlets face a problem in establishment due to a high degree of contamination in the medium to reduce contamination chemicals were tested for sterilisation of the explant. Sodium hypochlorite (5.0 percent v/v) in combination with mercuric chloride (0.5 percent w/v) resulted in the highest response. Explant establishment was dependent on their lengths as well as the plantlets from where the explants were derived. Explants of length 1.5-2.0 cm derived from plantlets having a length of 10-20 cm showed a higher rate of growth under culture conditions. Bud proliferation in explant was achieved in the establisment medium supplemented with BAP (6-benzylaminopurine) (5-10mg per l).
Additional Metadata
| Item Type: | Article |
|---|---|
| Additional Information: | Summaries (En Ms) |
| AGROVOC Term: | CALAMUS (PALMAE) |
| AGROVOC Term: | IN VITRO CULTURE |
| AGROVOC Term: | WOODY PLANTS |
| AGROVOC Term: | SHRUBS |
| AGROVOC Term: | TROPICAL RAIN FORESTS |
| AGROVOC Term: | PLANT PROPAGATION |
| AGROVOC Term: | STERILIZATION CALAMUS (PALMAE) |
| AGROVOC Term: | CULTIVO IN VITRO |
| AGROVOC Term: | PLANTAS LENOSAS |
| AGROVOC Term: | ARBUSTOS |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:52 |
| URI: | http://webagris.upm.edu.my/id/eprint/17635 |
Actions (login required)
 |
View Item |
