Production of elite planting materials of sentang (Azadirachta excelsa L.) via in vitro culture technique


Citation

Liew T.K., . and Chan L.K., . and Teo Chris K.H., . Production of elite planting materials of sentang (Azadirachta excelsa L.) via in vitro culture technique. pp. 337-343. ISSN 0126-575X

Abstract

Eight superior clones of Azadirachta excelsa L. were identified from 150 six months old sentang seedlings population using a programme created from the PASCAL programming language based on their height and diameter. Axillary bud of the superior clones cultured on Murashige and Skoog (MS) medium supplemented with 1.0 mg per litre BA 0.5 mg per litre NAA produced the most number of shoots (eight shoots per explant). In vitro shoots cultured on MS medium 0.5 mg per litre NAA 1.0 mg per litre IBA induced 55 percent of the shoots to root. Eighty per cent of the rooted shoots survived after being acclimatised for four weeks and transplanted to the soil.


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Abstract

Eight superior clones of Azadirachta excelsa L. were identified from 150 six months old sentang seedlings population using a programme created from the PASCAL programming language based on their height and diameter. Axillary bud of the superior clones cultured on Murashige and Skoog (MS) medium supplemented with 1.0 mg per litre BA 0.5 mg per litre NAA produced the most number of shoots (eight shoots per explant). In vitro shoots cultured on MS medium 0.5 mg per litre NAA 1.0 mg per litre IBA induced 55 percent of the shoots to root. Eighty per cent of the rooted shoots survived after being acclimatised for four weeks and transplanted to the soil.

Additional Metadata

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Item Type: Article
Additional Information: Summary (En)
AGROVOC Term: AZADIRACHTA
AGROVOC Term: TIMBER TREES
AGROVOC Term: PLANT PROPAGATION
AGROVOC Term: IN VITRO CULTURE
AGROVOC Term: BUDS
AGROVOC Term: STEMS
AGROVOC Term: PLANTING STOCK
AGROVOC Term: PLANTING
AGROVOC Term: SEEDLINGS
AGROVOC Term: FOREST PLANTATIONS
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:52
URI: http://webagris.upm.edu.my/id/eprint/17863

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