Antioxidant activity and metabolite changes in Centella asiatica with different drying methods using FTIR- and quantitative HPLC-based metabolomics

Nomi, A. G. and Handayani, H. and Khuluk, R. H. and Karomah, A. H. and Wulansari, L. and Yuliana, N. D. and Rohaeti, E. and Rafi, M. (2024) Antioxidant activity and metabolite changes in Centella asiatica with different drying methods using FTIR- and quantitative HPLC-based metabolomics. International Food Research Journal (Malaysia), 31. pp. 228-238. ISSN 2231 7546

Centella asiatica, known as Indian or Asiatic pennywort, is consumed raw as salad or used as a brain tonic, treatment of Alzheimer’s disease, and memory improvement. Differences in the drying method will lead to different levels of phytochemical profile and biological activity. Therefore, the present work aimed to investigate the Fourier transforms infrared (FTIR) spectra fingerprint profiles, HPLC analysis of four bioactive compounds, and antioxidant activity of C. asiatica samples exposed to various drying methods, including air-, oven-, and sun-drying. Results showed that all samples had identical FTIR spectra patterns, but there were differences in the absorbance intensities at 1692 and 1634 cm-¹ showing the effect of drying methods on the content of extracts’ bioactive compounds. These differences were analysed by chemometrics namely principal components analysis (PCA), and groupings were shown for the three samples. Based on the IC₅₀ values, ovendrying (OD) had the highest antioxidant activity, followed by sun-drying (SD) and airdrying (AD), with IC50 values of 52.25, 94.18, and 99.29 μg/mL, respectively. HPLC analysis showed that OD had a higher percentage for madecassoside and asiaticoside with values of 0.86 and 0.96%, respectively, compared to SD and AD. Meanwhile, AD had the highest content of madecassic and asiatic acids, with values of 0.50 and 0.48%. The absorbance and antioxidant activity data for the three C. asiatica extracts were analysed for the correlation using an orthogonal partial least square. Results showed that at 1006 - 989 cm-¹, it positively correlated with antioxidant activity, and could be identified as the C–O functional group of alcohol and phenol.

Full text available from: Official URL: http://www.ifrj.upm.edu.my/31%20(01)%202024/20%20-...

Abstract

Centella asiatica, known as Indian or Asiatic pennywort, is consumed raw as salad or used as a brain tonic, treatment of Alzheimer’s disease, and memory improvement. Differences in the drying method will lead to different levels of phytochemical profile and biological activity. Therefore, the present work aimed to investigate the Fourier transforms infrared (FTIR) spectra fingerprint profiles, HPLC analysis of four bioactive compounds, and antioxidant activity of C. asiatica samples exposed to various drying methods, including air-, oven-, and sun-drying. Results showed that all samples had identical FTIR spectra patterns, but there were differences in the absorbance intensities at 1692 and 1634 cm-¹ showing the effect of drying methods on the content of extracts’ bioactive compounds. These differences were analysed by chemometrics namely principal components analysis (PCA), and groupings were shown for the three samples. Based on the IC₅₀ values, ovendrying (OD) had the highest antioxidant activity, followed by sun-drying (SD) and airdrying (AD), with IC50 values of 52.25, 94.18, and 99.29 μg/mL, respectively. HPLC analysis showed that OD had a higher percentage for madecassoside and asiaticoside with values of 0.86 and 0.96%, respectively, compared to SD and AD. Meanwhile, AD had the highest content of madecassic and asiatic acids, with values of 0.50 and 0.48%. The absorbance and antioxidant activity data for the three C. asiatica extracts were analysed for the correlation using an orthogonal partial least square. Results showed that at 1006 - 989 cm-¹, it positively correlated with antioxidant activity, and could be identified as the C–O functional group of alcohol and phenol.

[error in script]

Item Type:	Article
AGROVOC Term:	medicinal plants
AGROVOC Term:	antioxidants
AGROVOC Term:	biochemical compounds
AGROVOC Term:	research
AGROVOC Term:	drying
AGROVOC Term:	spectroscopy
AGROVOC Term:	spectral analysis
AGROVOC Term:	chemical composition
AGROVOC Term:	bioactive compounds
AGROVOC Term:	phytochemicals
Geographical Term:	Indonesia
Uncontrolled Keywords:	antioxidant, Centella asiatica, chemometrics, drying method, FTIR, HPLC
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	29 Apr 2025 01:30
Last Modified:	29 Apr 2025 01:30
URI:	http://webagris.upm.edu.my/id/eprint/1827

Actions (login required)

View Item