Production of biosurfactant using Bacillus subtilis Natto fermentation

Yew, Seng Leow and Norhafizah Abdullah and Dayang Radiah Awang Biak and Nur Syakina Jamali and Rozita Rosli and Huey, Fang Teh (2023) Production of biosurfactant using Bacillus subtilis Natto fermentation. Pertanika Journal of Science & Technology (Malaysia), 31 (2). pp. 709-728. ISSN 2231-8526

Biosurfactants are microbial amphiphiles produced as primary metabolites by varieties of microorganisms. They are preferred over chemically derived surfactants owing to their intrinsic properties, such as superior environmental compatibility, biodegradability, anti-inflammatory and antimicrobial activity, and higher tolerance towards extreme environmental conditions such as temperature, salinity, and pH levels. However, commercial production of biosurfactants is still lacking. The main reason for this is the low yields obtained from fermentation processes, which causes them to be unable to compete compared to chemical surfactants. The present study conducted a one-factor-at-a-time (OFAT) analysis on fermentation conditions to enhance biosurfactant yield from a probiotic strain, Bacillus subtilis Natto. The fermentation was conducted by varying parameters such as nitrogen source, vegetable oils, inoculum size, amino acids, and pH of the fermentation medium. Results showed a significant improvement of 45% in biosurfactant production from B. subtilis Natto when the initial pH of the fermentation medium was adjusted to pH 6.8, urea as the nitrogen source, inoculum size of 6% v/v and the addition of palm olein at a concentration of 2% v/v as a substrate in the fermentation medium.

Full text available from: Official URL: http://www.pertanika.upm.edu.my/resources/files/Pe...

Abstract

Biosurfactants are microbial amphiphiles produced as primary metabolites by varieties of microorganisms. They are preferred over chemically derived surfactants owing to their intrinsic properties, such as superior environmental compatibility, biodegradability, anti-inflammatory and antimicrobial activity, and higher tolerance towards extreme environmental conditions such as temperature, salinity, and pH levels. However, commercial production of biosurfactants is still lacking. The main reason for this is the low yields obtained from fermentation processes, which causes them to be unable to compete compared to chemical surfactants. The present study conducted a one-factor-at-a-time (OFAT) analysis on fermentation conditions to enhance biosurfactant yield from a probiotic strain, Bacillus subtilis Natto. The fermentation was conducted by varying parameters such as nitrogen source, vegetable oils, inoculum size, amino acids, and pH of the fermentation medium. Results showed a significant improvement of 45% in biosurfactant production from B. subtilis Natto when the initial pH of the fermentation medium was adjusted to pH 6.8, urea as the nitrogen source, inoculum size of 6% v/v and the addition of palm olein at a concentration of 2% v/v as a substrate in the fermentation medium.

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Item Type:	Article
AGROVOC Term:	Bacillus subtilis
AGROVOC Term:	microorganisms
AGROVOC Term:	biosurfactants
AGROVOC Term:	fermentation
AGROVOC Term:	nitrogen content
AGROVOC Term:	parameters
AGROVOC Term:	statistical methods
AGROVOC Term:	optimization methods
AGROVOC Term:	plant oils
AGROVOC Term:	probiotics
Geographical Term:	Malaysia
Uncontrolled Keywords:	B. subtilis Natto, biosurfactant production, fermentation, OFAT analysis
Depositing User:	Ms. Azariah Hashim
Date Deposited:	25 Nov 2024 04:22
Last Modified:	27 Jan 2025 02:28
URI:	http://webagris.upm.edu.my/id/eprint/1886

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