Citation
Normah M.N., . and Chin H.F., . and Hor Y.L., . Desiccation and cryopreservation of embryonic axes of Hevea brasiliensis Mue ll.-Arg. pp. 299-303. ISSN 0126-6128
Abstract
Hevea embryonic axes were desiccated for a period of 1-5 hours and the moisture content was determined at the end of each hour of desiccation. Another set of embryonic axes were aseptically desiccated for the same period before they were cryopreserved for 16 hours by direct immersion in liquid nitrogen (-196 degrees C). At a moisture content between 14-20 (desiccation for 2-5 hours) 20-69 of the embryonic axes survived cryopreservation and formed seedlings with normal roots and shoots when cultured in vitro. Abnormalities were detected in some seedlings however hence refinement of the technique was needed
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Abstract
Hevea embryonic axes were desiccated for a period of 1-5 hours and the moisture content was determined at the end of each hour of desiccation. Another set of embryonic axes were aseptically desiccated for the same period before they were cryopreserved for 16 hours by direct immersion in liquid nitrogen (-196 degrees C). At a moisture content between 14-20 (desiccation for 2-5 hours) 20-69 of the embryonic axes survived cryopreservation and formed seedlings with normal roots and shoots when cultured in vitro. Abnormalities were detected in some seedlings however hence refinement of the technique was needed
Additional Metadata
| Item Type: | Article |
|---|---|
| Additional Information: | Ill.; 1 table; 12 ref. Summaries (En Malay) |
| AGROVOC Term: | HEVEA BRASILIENSIS |
| AGROVOC Term: | SECADO |
| AGROVOC Term: | VIABILIDAD DE LA SEMILLA |
| AGROVOC Term: | EMBRIONES VEGETALES/ CONTENIDO DE HUMEDAD |
| AGROVOC Term: | PRESERVACION |
| AGROVOC Term: | FISIOLO0841GIA VEGETAL |
| AGROVOC Term: | CONGELA |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:54 |
| URI: | http://webagris.upm.edu.my/id/eprint/19378 |
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