Effect of pre-treatment methods on the extractability of Christia vespertilionis by supercritical carbon dioxide

Izni Atikah Abd Hamid, . and Najla Laazizi, . and Ana Najwa Mustapa, . and Norazah Abd Rahman, . (2023) Effect of pre-treatment methods on the extractability of Christia vespertilionis by supercritical carbon dioxide. Pertanika Journal of Science & Technology (Malaysia), 31 (5). 2311 -2328. ISSN 2231-8526

Christia vespertilionis is a medicinal herb traditionally used as a complementary and alternative medicine to treat cancer and malaria. This study investigated the effect of pre-treatments of the Christia vespertilionis plant on supercritical CO₂ extraction yield and solubility. Four pre-treatments were studied: drying and grinding, doping with absolute ethanol (99%) and 80% (v/v) of ethanol/water, and microwave pre-treatment. The supercritical CO₂ extraction was conducted at a constant 13.8 MPa, 40℃ with 24 mL/min flow rate in 40 min of extraction time. It was found that the dried sample after drying and grinding pre-treatment produced the highest yield of 4.56 mg/g, whereas the lowest yield was obtained for the fresh leaves’ samples treated with microwave irradiation (1.26 mg/g). Doping techniques with absolute ethanol and 80% (v/v) were comparable in the 2.64 to 2.94 mg/g. GCMS results revealed that Christia vespertilionis extract comprises antioxidants, mainly phytol, limonene, and other medicinal compounds such as α-monolaurin and l-ascorbyl 2,6-dipalmitate. This study indicates that adding co-solvent was not the primary technique in supercritical CO₂ extraction to increase the extractability of compounds of interest from plant matrices.

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Abstract

Christia vespertilionis is a medicinal herb traditionally used as a complementary and alternative medicine to treat cancer and malaria. This study investigated the effect of pre-treatments of the Christia vespertilionis plant on supercritical CO₂ extraction yield and solubility. Four pre-treatments were studied: drying and grinding, doping with absolute ethanol (99%) and 80% (v/v) of ethanol/water, and microwave pre-treatment. The supercritical CO₂ extraction was conducted at a constant 13.8 MPa, 40℃ with 24 mL/min flow rate in 40 min of extraction time. It was found that the dried sample after drying and grinding pre-treatment produced the highest yield of 4.56 mg/g, whereas the lowest yield was obtained for the fresh leaves’ samples treated with microwave irradiation (1.26 mg/g). Doping techniques with absolute ethanol and 80% (v/v) were comparable in the 2.64 to 2.94 mg/g. GCMS results revealed that Christia vespertilionis extract comprises antioxidants, mainly phytol, limonene, and other medicinal compounds such as α-monolaurin and l-ascorbyl 2,6-dipalmitate. This study indicates that adding co-solvent was not the primary technique in supercritical CO₂ extraction to increase the extractability of compounds of interest from plant matrices.

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Item Type:	Article
AGROVOC Term:	carbon dioxide
AGROVOC Term:	medicinal plants
AGROVOC Term:	sampling
AGROVOC Term:	drying
AGROVOC Term:	supercritical fluid extraction
AGROVOC Term:	research data
Geographical Term:	Malaysia
Uncontrolled Keywords:	Christia vespertilionis
Depositing User:	Ms. Azariah Hashim
Date Deposited:	20 Jan 2025 02:37
Last Modified:	27 Jan 2025 03:02
URI:	http://webagris.upm.edu.my/id/eprint/1978

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