Conditions influencing in vitro conidia production in the Malaysian isolate of Peronosclerospora sorghi

Ahmad I.B., . and Lopez D.O., . Conditions influencing in vitro conidia production in the Malaysian isolate of Peronosclerospora sorghi. pp. 93-99. ISSN 0126-8643

Production of conidia on incubated leaf pieces harvested from diseased plants was influenced by temperature relative humidity (RH) and light/darkness. The minimum and maximum temperatures for sporulation were 17 degrees and 29 degrees C respectively. The optimum temperature for sporulation was 21-23 degrees C with spore counts of 19.33 x 10 to the power of 3 - 20.2 x 10 to the power of 3 spores per cm 2 of infected tissue were recorded. The fungus sporulated most abundantly at 100 percent RH but showed drastic reduction in spore numbers of lower RH. The minimum RH for sporulation was 90 percent. The number of spores produced varied inversely with light or dark conditions and intensity of light. Spore production occurred only when the leaves were incubated in the dark or exposed to 8 hours of low light intensity which was provided by a fluorescent light source placed at 400 cm (equivalent to 56 lux) and 600 cm (24 lux) for 8 hours or placed in the dark.

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Abstract

Production of conidia on incubated leaf pieces harvested from diseased plants was influenced by temperature relative humidity (RH) and light/darkness. The minimum and maximum temperatures for sporulation were 17 degrees and 29 degrees C respectively. The optimum temperature for sporulation was 21-23 degrees C with spore counts of 19.33 x 10 to the power of 3 - 20.2 x 10 to the power of 3 spores per cm 2 of infected tissue were recorded. The fungus sporulated most abundantly at 100 percent RH but showed drastic reduction in spore numbers of lower RH. The minimum RH for sporulation was 90 percent. The number of spores produced varied inversely with light or dark conditions and intensity of light. Spore production occurred only when the leaves were incubated in the dark or exposed to 8 hours of low light intensity which was provided by a fluorescent light source placed at 400 cm (equivalent to 56 lux) and 600 cm (24 lux) for 8 hours or placed in the dark.

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Item Type:	Article
Additional Information:	3 tables; 27 ref. Summary (En Ms)
AGROVOC Term:	PERONOSCLEROSPORA
AGROVOC Term:	ESPORAS/ ESPORULACION
AGROVOC Term:	EXPERIMENTACION IN VITRO
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:55
URI:	http://webagris.upm.edu.my/id/eprint/19972

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