Citation
Arokiaraj P., . and Wan Abdul Rahaman W.Y., . Agrobacterium-mediated transformation of Hevea cells derived from in vitro and in vivo seedling cultures. pp. 55-61. ISSN 0127-7065
Abstract
Tumour tissue was produced on stems of in vitro and in vivo propagated rubber (Hevea brasiliensis) seedlings of PB 5/51 infected with a strain of Agrobacterium tumefaciens 541 (isolated by the Rubber Research Institute of Malaysia in 1971). The tumour tissues exhibited phytohormone-independent growth in tissue culture and produced octopine. In contrast controls inoculated with distilled water on seedlings did not produce crown gall. Octopine-utilising Agrobacterium strain 541 was distinguished from non-utilisers on a solid medium containing octopine as the only added nitrogen source with bromothymolblue (BTB) as the pH indicator. On blue BTB plates this strain formed orange-yellow colonies while those formed by non-utilisers were translucent. This process of cell transformation may facilitate the transfer of genes of economic importance to rubber.
Download File
Full text available from:
|
Abstract
Tumour tissue was produced on stems of in vitro and in vivo propagated rubber (Hevea brasiliensis) seedlings of PB 5/51 infected with a strain of Agrobacterium tumefaciens 541 (isolated by the Rubber Research Institute of Malaysia in 1971). The tumour tissues exhibited phytohormone-independent growth in tissue culture and produced octopine. In contrast controls inoculated with distilled water on seedlings did not produce crown gall. Octopine-utilising Agrobacterium strain 541 was distinguished from non-utilisers on a solid medium containing octopine as the only added nitrogen source with bromothymolblue (BTB) as the pH indicator. On blue BTB plates this strain formed orange-yellow colonies while those formed by non-utilisers were translucent. This process of cell transformation may facilitate the transfer of genes of economic importance to rubber.
Additional Metadata
| Item Type: | Article |
|---|---|
| Additional Information: | 5 plates; 1 table 12 ref. Summary (En) |
| AGROVOC Term: | HEVEA BRASILIENSIS |
| AGROVOC Term: | AGROBACTERIUM TUMEFACIENS |
| AGROVOC Term: | AGALLAS/ EXPERIMENTACION IN VIVO |
| AGROVOC Term: | EXPERIMENTACION IN VITRO |
| AGROVOC Term: | TRANSFORMACION GENETICA |
| Depositing User: | Ms. Norfaezah Khomsan |
| Last Modified: | 24 Apr 2025 05:55 |
| URI: | http://webagris.upm.edu.my/id/eprint/20075 |
Actions (login required)
 |
View Item |
