Effect of dehydration on freezing characteristics and survival in liquid nitrogen of three recalcitrant seeds

Hor Y.L., . and Stanwood P.C., . and Chin H.F., . Effect of dehydration on freezing characteristics and survival in liquid nitrogen of three recalcitrant seeds. pp. 309-314. ISSN 0126-6128

The recalcitrant seeds rambutan (Nephelium lappaceum) durian (Durio zibethinus) and cempedak (Artocarpus integer) have a high critical moisture content (below which rapid loss of viability occurs) of 27.0 percent 26.0 percent and 37.9 percent respectively. The critical moisture contents for embryos were higher at 39.0 percent for rambutan 53.9 percent for durian and 43.2 percent for cempedak. Differential thermal analysis of the embryos confirmed that their threshold moistures (below which there is no freezable water) were lower than their critical moisture. The threshold moistures for rambutan durian and cempedak embryos were approximately 30 percent 32 percent and 33 percent respectively. It is suggested that unsuccessful attempts at cryopreservation of embryos of recalcitrant seeds in the past may be due to the absence of a safe window between the high critical moisture content and the threshold moisture. This results in freezing injury at the higher moistures and dehydration injury at the lower moistures. Potential techniques to overcome this and improve cryopreservation of recalcitrant seed embryos are discussed.

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Abstract

The recalcitrant seeds rambutan (Nephelium lappaceum) durian (Durio zibethinus) and cempedak (Artocarpus integer) have a high critical moisture content (below which rapid loss of viability occurs) of 27.0 percent 26.0 percent and 37.9 percent respectively. The critical moisture contents for embryos were higher at 39.0 percent for rambutan 53.9 percent for durian and 43.2 percent for cempedak. Differential thermal analysis of the embryos confirmed that their threshold moistures (below which there is no freezable water) were lower than their critical moisture. The threshold moistures for rambutan durian and cempedak embryos were approximately 30 percent 32 percent and 33 percent respectively. It is suggested that unsuccessful attempts at cryopreservation of embryos of recalcitrant seeds in the past may be due to the absence of a safe window between the high critical moisture content and the threshold moisture. This results in freezing injury at the higher moistures and dehydration injury at the lower moistures. Potential techniques to overcome this and improve cryopreservation of recalcitrant seed embryos are discussed.

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Item Type:	Article
Additional Information:	4 ill.; 1 table; 13 ref. Summary (En Ms)
AGROVOC Term:	NEPHELIUM LAPPACEUM
AGROVOC Term:	DURIO ZIBETHINUS
AGROVOC Term:	ARTOCARPUS HETEROPHYLLUS
AGROVOC Term:	SEMILLAS/ CONGELACION
AGROVOC Term:	PRESERVACION
AGROVOC Term:	CONTENIDO DE HUMEDAD
Depositing User:	Ms. Norfaezah Khomsan
Last Modified:	24 Apr 2025 05:56
URI:	http://webagris.upm.edu.my/id/eprint/20295

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