Control of sclerotium seedling blight of barley by using seeds coated with Trichoderma spp. and Bacillus sp.


Citation

Chamswarng C., . and Gesnara W., . and Korpraditskul, . Control of sclerotium seedling blight of barley by using seeds coated with Trichoderma spp. and Bacillus sp. pp. 69-75. ISSN 0127-6883

Abstract

The efficacy of six isolates of Trichoderma spp. and one isolate of Bacillus sp. for the control of seedling blight on barley (BRB 2) caused by Sclerotium rolfsii Sacc. was investigated under screenhouse conditions. Barley seeds were coated with spore/cell suspension alone or in combination with test isolates. Seeds coated with each spore suspension of T. harzianum (T1 T2) and Trichoderma sp. (T9) or as combinations of T. harzianum (T1) with Bacillus sp. (B1) T. aureoviride (T4) with Bacillus sp. (B1) or T. longibrachiatum (T7) were significantly protected from sclerotium infection with 30.0 25 45 30 27.5 and 25.0 more surviving plants than the control. These control capabilities were not significantly different from the carboxin when compared with the control. Twenty eight days after planting populations of these Trichoderma isolates in the rhizosphere of treated seeds were found ranging from 3.5 to 6.0 x 10 7 cfu/g soil. Replanting of surface-disinfested barley seeds in soil formerly planted with treated seeds (Trichoderma spp. and/or Bacillus) showed that numbers of surviving plants of all treatments except seeds treated with T. longibrachiatum (T7) and T. aureoviride (T4) in combination with Bacillus sp. (B) were significantly higher than the control and not significantly different from the carboxin treatment


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Abstract

The efficacy of six isolates of Trichoderma spp. and one isolate of Bacillus sp. for the control of seedling blight on barley (BRB 2) caused by Sclerotium rolfsii Sacc. was investigated under screenhouse conditions. Barley seeds were coated with spore/cell suspension alone or in combination with test isolates. Seeds coated with each spore suspension of T. harzianum (T1 T2) and Trichoderma sp. (T9) or as combinations of T. harzianum (T1) with Bacillus sp. (B1) T. aureoviride (T4) with Bacillus sp. (B1) or T. longibrachiatum (T7) were significantly protected from sclerotium infection with 30.0 25 45 30 27.5 and 25.0 more surviving plants than the control. These control capabilities were not significantly different from the carboxin when compared with the control. Twenty eight days after planting populations of these Trichoderma isolates in the rhizosphere of treated seeds were found ranging from 3.5 to 6.0 x 10 7 cfu/g soil. Replanting of surface-disinfested barley seeds in soil formerly planted with treated seeds (Trichoderma spp. and/or Bacillus) showed that numbers of surviving plants of all treatments except seeds treated with T. longibrachiatum (T7) and T. aureoviride (T4) in combination with Bacillus sp. (B) were significantly higher than the control and not significantly different from the carboxin treatment

Additional Metadata

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Item Type: Article
Additional Information: 2 tables 10 ref. Summary (En Ms)
AGROVOC Term: CEBADA
AGROVOC Term: TIZON
AGROVOC Term: SCLEROTIUM
AGROVOC Term: SEMILLA
AGROVOC Term: TRICHODERMA
AGROVOC Term: BACILLUS
Depositing User: Ms. Norfaezah Khomsan
Last Modified: 24 Apr 2025 05:56
URI: http://webagris.upm.edu.my/id/eprint/20637

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