Antilisterial activity of broccoli stems (Brassica oleracea) by flow cytometry

Alencar S. M., . and Corrêa C. B., . and Porto E., . and Corrêa C. B., . and Martin J. G. P., . Antilisterial activity of broccoli stems (Brassica oleracea) by flow cytometry. pp. 395-399. ISSN 22317546

Studies have demonstrated the presence of antimicrobial compounds in vegetal species. However natural components can be found not only in raw materials but also in agro-industrial wastes. Their reuse as source of natural preservatives in food and beverage industry can promote sustainable practices since environmental risks caused by its discard are reduced. The antimicrobial activity of broccoli stems (Brassica oleracea) aqueous extracts (1:20 w/v) against Listeria monocytogenes was evaluated by detection of Minimal Inhibition Concentration (MIC) and flow cytometry analysis. The chemical composition of major compounds was detected by Gas Chromatography with Mass Spectrometry (CG-MS). Broccoli stems extract showed antimicrobial activity against L. monocytogenes which MIC was 102.4 mg/mL. Analysis by CG-MS allowed the identification of organic acids as ascorbic and malic acids and phenolic compounds as sinapinic ferulic and caffeic acids. The use of flow cytometry to evaluate the antimicrobial activity of the extracts was very suitable enabling to infer their action mechanisms. In face of the difficulty to avoid the food contamination by this pathogen the study of vegetal residues as source of natural preservatives in food and beverage industry is very promising.

Full text available from: Official URL: http://ifrj.upm.edu.my/21%20(01)%202014/56%20IFRJ%...

Abstract

Studies have demonstrated the presence of antimicrobial compounds in vegetal species. However natural components can be found not only in raw materials but also in agro-industrial wastes. Their reuse as source of natural preservatives in food and beverage industry can promote sustainable practices since environmental risks caused by its discard are reduced. The antimicrobial activity of broccoli stems (Brassica oleracea) aqueous extracts (1:20 w/v) against Listeria monocytogenes was evaluated by detection of Minimal Inhibition Concentration (MIC) and flow cytometry analysis. The chemical composition of major compounds was detected by Gas Chromatography with Mass Spectrometry (CG-MS). Broccoli stems extract showed antimicrobial activity against L. monocytogenes which MIC was 102.4 mg/mL. Analysis by CG-MS allowed the identification of organic acids as ascorbic and malic acids and phenolic compounds as sinapinic ferulic and caffeic acids. The use of flow cytometry to evaluate the antimicrobial activity of the extracts was very suitable enabling to infer their action mechanisms. In face of the difficulty to avoid the food contamination by this pathogen the study of vegetal residues as source of natural preservatives in food and beverage industry is very promising.

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Item Type:	Article
AGROVOC Term:	Broccoli
AGROVOC Term:	Brassica oleracea
AGROVOC Term:	Flow cytometry (cells)
AGROVOC Term:	Antimicrobial properties
AGROVOC Term:	Industrial wastes
AGROVOC Term:	Preservatives
AGROVOC Term:	Listeria monocytogenes
AGROVOC Term:	Gas chromatography
AGROVOC Term:	Mass spectrometry
AGROVOC Term:	Antimicrobials
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:26
URI:	http://webagris.upm.edu.my/id/eprint/21054

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