Citation
Yusof Ibrahim, . and Tan Gek Kiang, . and Cheong Chee Kong, . Laboratory and field evaluation of selected isolates of Metarhizium anisopliae and Beauveria bassiana against the leaf beetle Epilachna indica Mulsant. 15-. ISSN 0128-9322
Abstract
Six isolates of selected entomopathogenic fungi were tested for their efficacy against the eggs larvae and adults of the ladybird beetle Epilachna indica (Mulsant) under an ambient environment of 28 20C 85 15 RH and 12 h photoperiod. All Metarhizium anisopliae were isolates were infective against the eggs larvae and adults of E. indica. Exposure to a suspension of 1 x 108 conidia ml-1 resulted in 99.2 - 91.5 infection on the eggs 71.7 - 65.0 on the 1st instar larvae and 86.7 - 76.7 on the adults seven days after treatment (7-DAT). Probit analysis showed significantly positive relationship between log dosage and probit mortality values with isolate MaOr providing the best estimated median effective concentration (EC50) of 9.268 x 103 conidia ml-1 against the eggs and 2.855 x 106 conidia ml-1 against the 1st instar larvae with estimated median lethal time (LT5o) value of 4.9 days while isolate MaPs gave the best EC5o value of 1.486 x 10 5 conidia ml-1 with LT5o pt value of 7.0 days against the adults. Infectivity by Beauveria bassiana was rather weak with the highest infection (54.5) on the eggs being inflicted by isolate BbPc while the highest against the 1 st instar larvae (58.3) by isolate BbGc and that against the adults (40.0) by isolate BbPc. In general isolates of M. anisopliae inflicted the highest mortality against the eggs larvae and adults of E. indica. Field experiment showed M. anisopliae (MaPs) and Deltamethrin were equally effective and inflicted significantly higher larval mortality (75.0 and 98.0 respectively) and lesser leaf damage (81.4 and 78.6 respectively) compared to B. bassiana (BbGc) and the control five days after the last spray.
Download File
Full text available from:
|
Abstract
Six isolates of selected entomopathogenic fungi were tested for their efficacy against the eggs larvae and adults of the ladybird beetle Epilachna indica (Mulsant) under an ambient environment of 28 20C 85 15 RH and 12 h photoperiod. All Metarhizium anisopliae were isolates were infective against the eggs larvae and adults of E. indica. Exposure to a suspension of 1 x 108 conidia ml-1 resulted in 99.2 - 91.5 infection on the eggs 71.7 - 65.0 on the 1st instar larvae and 86.7 - 76.7 on the adults seven days after treatment (7-DAT). Probit analysis showed significantly positive relationship between log dosage and probit mortality values with isolate MaOr providing the best estimated median effective concentration (EC50) of 9.268 x 103 conidia ml-1 against the eggs and 2.855 x 106 conidia ml-1 against the 1st instar larvae with estimated median lethal time (LT5o) value of 4.9 days while isolate MaPs gave the best EC5o value of 1.486 x 10 5 conidia ml-1 with LT5o pt value of 7.0 days against the adults. Infectivity by Beauveria bassiana was rather weak with the highest infection (54.5) on the eggs being inflicted by isolate BbPc while the highest against the 1 st instar larvae (58.3) by isolate BbGc and that against the adults (40.0) by isolate BbPc. In general isolates of M. anisopliae inflicted the highest mortality against the eggs larvae and adults of E. indica. Field experiment showed M. anisopliae (MaPs) and Deltamethrin were equally effective and inflicted significantly higher larval mortality (75.0 and 98.0 respectively) and lesser leaf damage (81.4 and 78.6 respectively) compared to B. bassiana (BbGc) and the control five days after the last spray.
Additional Metadata
Item Type: | Article |
---|---|
Additional Information: | 4 tables |
AGROVOC Term: | ENTOMOGENOUS FUNGI |
AGROVOC Term: | EPILACHNA |
AGROVOC Term: | METARHIZIUM ANISOPLIAE |
AGROVOC Term: | BEAUVERIA BASSIANA |
AGROVOC Term: | INOCULATION |
AGROVOC Term: | BIOASSAYS |
AGROVOC Term: | MICROBIAL PESTICIDES |
AGROVOC Term: | MALAYSIA HONGOS ENTOMOPATOGENOS |
AGROVOC Term: | EPILACHNA |
AGROVOC Term: | METARHIZIUM ANISOPLIAE |
Depositing User: | Ms. Suzila Mohamad Kasim |
Last Modified: | 24 Apr 2025 06:26 |
URI: | http://webagris.upm.edu.my/id/eprint/21185 |
Actions (login required)
![]() |
View Item |