Ribosomal DNA analysis of marine microbes associated with toxin-producing Pyrodinium bahamense var. compressum (Bhm) a harmful algal bloom species.

Chin G. J. W. L., . and Teoh P. L., . and Kumar S. V., . and Anton A., . Ribosomal DNA analysis of marine microbes associated with toxin-producing Pyrodinium bahamense var. compressum (Bhm) a harmful algal bloom species. pp. 179-188. ISSN 0126-6128

Blooms ofthe toxic alga Pyrodinillm bahamense var. compressum (Bohm) have become a problem in Malaysia over the past three decades. The alga is a causative agent of paralytic shellfish poisoning a potentially fatal neurological disorder. Past research suggest that bacteria-algae association may play a direct or indirect role in toxin production. As such ribosomal DNA-based restriction enzyme analysis for the identification of bacteria associated with Pyrodinillm sps. was undertaken. A total of 16 bacterial isolates were successfully obtained from the clonal cultures of Pyrodinium sps. The diversity of the eJl.tracellular bacteria associated with Pyrodinium bahamense var. compressum was limited to the Phyla Proteobacteria and Actinobacteria. The major bacterial species identified included Alcanivorax spp. and Hyphomonas spp. whereas Kocuria spp. Nesterenkonia spp. Alteromonas spp. Roseobacter spp. Xanthomonas spp. and Acinetobacter spp. were identified as minor isolates. The identified bacterium Hyphomonas spp. exhibited high sequence identity with an unknown bacterium strain SCRlPPS_739 in the GenBank database that is known to be associated with toxic and non-toxic dinoflagellates Alexandrillm spp. and Scrippsiella trochoidea respectively.

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Abstract

Blooms ofthe toxic alga Pyrodinillm bahamense var. compressum (Bohm) have become a problem in Malaysia over the past three decades. The alga is a causative agent of paralytic shellfish poisoning a potentially fatal neurological disorder. Past research suggest that bacteria-algae association may play a direct or indirect role in toxin production. As such ribosomal DNA-based restriction enzyme analysis for the identification of bacteria associated with Pyrodinillm sps. was undertaken. A total of 16 bacterial isolates were successfully obtained from the clonal cultures of Pyrodinium sps. The diversity of the eJl.tracellular bacteria associated with Pyrodinium bahamense var. compressum was limited to the Phyla Proteobacteria and Actinobacteria. The major bacterial species identified included Alcanivorax spp. and Hyphomonas spp. whereas Kocuria spp. Nesterenkonia spp. Alteromonas spp. Roseobacter spp. Xanthomonas spp. and Acinetobacter spp. were identified as minor isolates. The identified bacterium Hyphomonas spp. exhibited high sequence identity with an unknown bacterium strain SCRlPPS_739 in the GenBank database that is known to be associated with toxic and non-toxic dinoflagellates Alexandrillm spp. and Scrippsiella trochoidea respectively.

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Item Type:	Article
Additional Information:	2 ills.; 28 ref.
AGROVOC Term:	Bacteria
AGROVOC Term:	Ribosomal rna
AGROVOC Term:	DNA
AGROVOC Term:	Microbes
AGROVOC Term:	Toxins
AGROVOC Term:	Algal blooms
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	26 Apr 2025 02:57
URI:	http://webagris.upm.edu.my/id/eprint/21194

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