Antioxidant antimicrobial activity and in-vitro cytotoxicity screening study of Pili nut oil

Maaruf A. G., . and Zarinah Z., . and Wong W. W. W., . and Xuebing X., . and Nazaruddin R., . Antioxidant antimicrobial activity and in-vitro cytotoxicity screening study of Pili nut oil. pp. 309-316. ISSN 22317546

Some vegetable oils contain natural antioxidants such as beta carotene and vitamin E namely alpha tocopherol. The objective of this study was to screening the value of -tocopherol -carotene antioxidant capacity antimicrobial activity and toxicological properties of roasted pili nut oil (RPNO) and unroasted pili nut oil (UPNO). The result showed that RPNO contained higher amount of vitamin E and less amount of beta carotene compared to UPNO. RPNO and UPNO scavenged DPPH radicals by 24.66 and 9.52 at concentration of 140 g/ml. The total phenolic compound (TPC) in UPNO and RPNO were about 19.96 0.52 mg/kg and 12.43 0.69 mg/kg respectively. It was observed that bacteria species exhibited different sensitivities towards RPNO UPNO Gentamycin Ampicillin and Chloramphenicol. Bacillus cereus 14570 was the most sensitive bacterium and all strains of Staphylococcus aureus tested were resistant against both samples RPNO and UPNO. An in vitro toxicological study based on the MTT (3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyl tetrazolium bromide) cytotoxicity assay was also performed. In vitro cytotoxicity indicated that both RPNO and UPNO had no effect against HeLa (cervical cancer cell) MCF-7 (breast cancer cell) and HT-29 (human colon adenocarcinoma cell) cell lines tested.

Full text available from: Official URL: http://ifrj.upm.edu.my/21%20(01)%202014/44%20IFRJ%...

Abstract

Some vegetable oils contain natural antioxidants such as beta carotene and vitamin E namely alpha tocopherol. The objective of this study was to screening the value of -tocopherol -carotene antioxidant capacity antimicrobial activity and toxicological properties of roasted pili nut oil (RPNO) and unroasted pili nut oil (UPNO). The result showed that RPNO contained higher amount of vitamin E and less amount of beta carotene compared to UPNO. RPNO and UPNO scavenged DPPH radicals by 24.66 and 9.52 at concentration of 140 g/ml. The total phenolic compound (TPC) in UPNO and RPNO were about 19.96 0.52 mg/kg and 12.43 0.69 mg/kg respectively. It was observed that bacteria species exhibited different sensitivities towards RPNO UPNO Gentamycin Ampicillin and Chloramphenicol. Bacillus cereus 14570 was the most sensitive bacterium and all strains of Staphylococcus aureus tested were resistant against both samples RPNO and UPNO. An in vitro toxicological study based on the MTT (3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyl tetrazolium bromide) cytotoxicity assay was also performed. In vitro cytotoxicity indicated that both RPNO and UPNO had no effect against HeLa (cervical cancer cell) MCF-7 (breast cancer cell) and HT-29 (human colon adenocarcinoma cell) cell lines tested.

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Item Type:	Article
AGROVOC Term:	Cytotoxicity
AGROVOC Term:	Vegetable oils
AGROVOC Term:	Antioxidants
AGROVOC Term:	Carotenes
AGROVOC Term:	Tocopherols
AGROVOC Term:	Antimicrobials
AGROVOC Term:	Bacteria
AGROVOC Term:	Gentamycin
AGROVOC Term:	Ampicillin
AGROVOC Term:	Chloramphenicol
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	26 Apr 2025 03:07
URI:	http://webagris.upm.edu.my/id/eprint/21289

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