Effects of extraction conditions on antioxidant properties of sapodilla fruit (Manilkara zapota)


Citation

Sia C. M., . and Yim H. S., . and Ang Y. K., . and Woo P. F., . and Khoo H. E., . Effects of extraction conditions on antioxidant properties of sapodilla fruit (Manilkara zapota). pp. 2065-2072. ISSN 22317546

Abstract

This study investigated the effects of different percentages of ethanol (0 - 100) extraction times (1 - 5 h) and temperatures (25 - 60C) on total phenolic content (TPC) and antioxidant activity (AA) of sapodilla pulp and peel. TPC was determined by Folin-Ciocalteu reagent method while AA was evaluated using 2 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay 2 2-azino-bis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay ferric reducing antioxidant power (FRAP) assay and -carotene bleaching (BCB) assay. Based on the optimal extraction conditions used sapodilla pulp extract had TPC of 3.89 mg GAE/g 63.20 of DPPH scavenging activity 4.30 of ABTS scavenging activity 19.17 of BCB activity and FRAP value of 15.24 mg TE/g; while its peel extract had TPC of 9.23 mg GAE/g 92.95 of DPPH scavenging activity 5.36 of ABTS scavenging activity 8.14 of BCB activity and 27.85 mg TE/g (FRAP value). Using the optimal extraction conditions for sapodilla pulp (40 ethanol as extraction solvent that extracted at 60C for 4 h) and sapodilla peel (80 ethanol and 2 h extraction time at 40C) highest antioxidants can be extracted from the pulp and peel.


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Abstract

This study investigated the effects of different percentages of ethanol (0 - 100) extraction times (1 - 5 h) and temperatures (25 - 60C) on total phenolic content (TPC) and antioxidant activity (AA) of sapodilla pulp and peel. TPC was determined by Folin-Ciocalteu reagent method while AA was evaluated using 2 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay 2 2-azino-bis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay ferric reducing antioxidant power (FRAP) assay and -carotene bleaching (BCB) assay. Based on the optimal extraction conditions used sapodilla pulp extract had TPC of 3.89 mg GAE/g 63.20 of DPPH scavenging activity 4.30 of ABTS scavenging activity 19.17 of BCB activity and FRAP value of 15.24 mg TE/g; while its peel extract had TPC of 9.23 mg GAE/g 92.95 of DPPH scavenging activity 5.36 of ABTS scavenging activity 8.14 of BCB activity and 27.85 mg TE/g (FRAP value). Using the optimal extraction conditions for sapodilla pulp (40 ethanol as extraction solvent that extracted at 60C for 4 h) and sapodilla peel (80 ethanol and 2 h extraction time at 40C) highest antioxidants can be extracted from the pulp and peel.

Additional Metadata

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Item Type: Article
AGROVOC Term: Extraction
AGROVOC Term: Antioxidants
AGROVOC Term: Sapodilla
AGROVOC Term: Manilkara zapota
AGROVOC Term: Ethanol
AGROVOC Term: Temperature profile
AGROVOC Term: Phenolic content
AGROVOC Term: Pulp
AGROVOC Term: Chemical reagents
AGROVOC Term: Bleaching
Geographical Term: Malaysia
Depositing User: Ms. Suzila Mohamad Kasim
Last Modified: 26 Apr 2025 03:07
URI: http://webagris.upm.edu.my/id/eprint/21309

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