Antioxidant properties and antiproliferative effect of brewers rice extract (temukut) on selected cancer cell lines

Tan B. L., . and Norhaizan M. E., . and Suhaniza H. J., . and Lai C. C., . and Norazalina S., . and Roselina K., . Antioxidant properties and antiproliferative effect of brewers rice extract (temukut) on selected cancer cell lines. pp. 2117-2124. ISSN 22317546

Temukut or brewers rice is a mixture of broken rice rice bran and rice germ. Extensive studies have been conducted on rice bran which possesses various health benefits. Temukut however has been less well studied. The present study aimed to investigate the antioxidant and growth inhibition properties of temukut extract using colon cancer (HT-29) ovary cancer (Caov-3) and liver cancer (HepG2) cell lines. The antioxidant activity was determined by the -carotene bleaching assay analysis of the DPPH radical scavenging capacity and a FRAP assay. The total phenolic compounds oryzanol vitamin E and phytic acid levels in temukut were also investigated. The antiproliferative activity was evaluated using the 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide (MTT) assay. There was a significant difference in the cytotoxicity of two types of temukut extract (water and methanol) for HT-29 and Caov-3 cells (p 0.05) but not for HepG2 cells. The HepG2 cell line is the least sensitive to temukut (IC50 55.30 g/mL) whereas the highest sensitivity was observed in Caov-3 cells (IC50 36.67 g/mL). No cytotoxic effect of temukut was observed on normal cells (BalBlc3T3). Although the content of the phytochemicals studied (total phenolic compounds vitamin E oryzanol and phytic acid) in temukut was lower than that in rice bran as has been previously reported the present study demonstrated temukuts potential to inhibit the proliferation of HT-29 Caov-3 and HepG2 cells.

Full text available from: Official URL: http://ifrj.upm.edu.my/20%20(05)%202013/11%20IFRJ%...

Abstract

Temukut or brewers rice is a mixture of broken rice rice bran and rice germ. Extensive studies have been conducted on rice bran which possesses various health benefits. Temukut however has been less well studied. The present study aimed to investigate the antioxidant and growth inhibition properties of temukut extract using colon cancer (HT-29) ovary cancer (Caov-3) and liver cancer (HepG2) cell lines. The antioxidant activity was determined by the -carotene bleaching assay analysis of the DPPH radical scavenging capacity and a FRAP assay. The total phenolic compounds oryzanol vitamin E and phytic acid levels in temukut were also investigated. The antiproliferative activity was evaluated using the 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide (MTT) assay. There was a significant difference in the cytotoxicity of two types of temukut extract (water and methanol) for HT-29 and Caov-3 cells (p 0.05) but not for HepG2 cells. The HepG2 cell line is the least sensitive to temukut (IC50 55.30 g/mL) whereas the highest sensitivity was observed in Caov-3 cells (IC50 36.67 g/mL). No cytotoxic effect of temukut was observed on normal cells (BalBlc3T3). Although the content of the phytochemicals studied (total phenolic compounds vitamin E oryzanol and phytic acid) in temukut was lower than that in rice bran as has been previously reported the present study demonstrated temukuts potential to inhibit the proliferation of HT-29 Caov-3 and HepG2 cells.

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Item Type:	Article
AGROVOC Term:	Antioxidants
AGROVOC Term:	Brewers grains
AGROVOC Term:	Cancer (disease)
AGROVOC Term:	Growth inhibition
AGROVOC Term:	Carotenes
AGROVOC Term:	Bleaching
AGROVOC Term:	Free radicals
AGROVOC Term:	Phenolic compounds
AGROVOC Term:	Vitamin e
AGROVOC Term:	Phytic acid
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:26
URI:	http://webagris.upm.edu.my/id/eprint/21315

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