Phenolic acid profile of oat cultivars, and their suppressive effect on intracellular reactive oxygen species

Manzoor, M. S. and Pasha, I. and Younas, S. and Zhu, M. and Hussain, R. and Arqam,, U. and Younis, N. and Chughtai, M. F. J. (2023) Phenolic acid profile of oat cultivars, and their suppressive effect on intracellular reactive oxygen species. International Food Research Journal (Malaysia), 30. pp. 87-95. ISSN 2231 7546

Oat (Avena sativa L.) has rich phenolic contents with nutritional and therapeutic health benefits. The objective of the present work was to perform a cell cytotoxicity assay of three South Asian oat cultivars to determine their suppressive effect on intracellular reactive oxygen species (ROS). Oat cultivars were firstly examined for total phenolic content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, and phenolic acid profiling through HPLC. Total phenolic contents of oat cultivars ranged from 167.57 to 198.41 mg GAE/100 g, and DPPH scavenging activity was IC50 = 18.81 to 13.18 mg/mL. Oat cultivars displayed average content of phenolic acids such as vanillic acid (0.33 µg/g), syringic acid (6.70 µg/g), caffeic acid (4.16 µg/g), ferulic acid (2.06 µg/g), p-coumaric acid (20.22 µg/g), with the highest being gallic acid (74.32 µg/g). Cell cytotoxicity assay of oat polyphenolic extracts revealed that 10 and 15 µg/mL concentrations had nonsignificant differences when compared with their respective control treatments. Oat extracts suppressed ROS in Caco-2-cells, with or without hydrogen peroxide stimulation from 65.20 - 86.13%, and 56.36 - 79.56% with 10 and 15 µg/mL concentrations, respectively. In conclusion, oat is a rich source of polyphenols which have strong antioxidant behaviour to suppress the ROS activity

Full text available from: Official URL: http://www.ifrj.upm.edu.my/30%20(01)%202023/06%20-...

Abstract

Oat (Avena sativa L.) has rich phenolic contents with nutritional and therapeutic health benefits. The objective of the present work was to perform a cell cytotoxicity assay of three South Asian oat cultivars to determine their suppressive effect on intracellular reactive oxygen species (ROS). Oat cultivars were firstly examined for total phenolic content, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity, and phenolic acid profiling through HPLC. Total phenolic contents of oat cultivars ranged from 167.57 to 198.41 mg GAE/100 g, and DPPH scavenging activity was IC50 = 18.81 to 13.18 mg/mL. Oat cultivars displayed average content of phenolic acids such as vanillic acid (0.33 µg/g), syringic acid (6.70 µg/g), caffeic acid (4.16 µg/g), ferulic acid (2.06 µg/g), p-coumaric acid (20.22 µg/g), with the highest being gallic acid (74.32 µg/g). Cell cytotoxicity assay of oat polyphenolic extracts revealed that 10 and 15 µg/mL concentrations had nonsignificant differences when compared with their respective control treatments. Oat extracts suppressed ROS in Caco-2-cells, with or without hydrogen peroxide stimulation from 65.20 - 86.13%, and 56.36 - 79.56% with 10 and 15 µg/mL concentrations, respectively. In conclusion, oat is a rich source of polyphenols which have strong antioxidant behaviour to suppress the ROS activity

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Item Type:	Article
AGROVOC Term:	oats
AGROVOC Term:	phenolic acids
AGROVOC Term:	reactive oxygen species
AGROVOC Term:	sampling
AGROVOC Term:	extraction
AGROVOC Term:	phenolic content
AGROVOC Term:	statistical methods
AGROVOC Term:	human health
Geographical Term:	Pakistan
Uncontrolled Keywords:	Avena sativa L,
Depositing User:	Nor Hasnita Abdul Samat
Date Deposited:	28 Oct 2024 02:07
Last Modified:	28 Oct 2024 06:53
URI:	http://webagris.upm.edu.my/id/eprint/214

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