Nutritional requirements for in vitro seed germination of 12 terrestrial lithophytic andepiphytic orchids

Seaton P. T., . and Wood S., . and Pritchard H. W., . and J. Nadarajan, . and Marks T. R., . Nutritional requirements for in vitro seed germination of 12 terrestrial lithophytic andepiphytic orchids. pp. 204-212. ISSN 0128-1283

Although numerous media have been developed to evaluate both orchid seed germination and further seedling development after storage few studies have attempted to relate nutritional requirements to life history traits. For a range of terrestrial lithophytic and epiphytic orchid species comparisons were made of germination on Knudson C with and without activated charcoal or banana powder Norstog and Phytamax„ media to represent variations in available nitrogen. Germination varied with maximum values ranging from just 9 for Prosthechea cochleata Platanthera sp. and Spathoglottis paulinae to 95 for Phragmapedium longifolium. Along with Paphiopedilumdelenatii Paphiopedilum philippinense and the epiphyte Guarianthe bowringiana P. longifolium germinated well on most media. Germination was significantly higher on Norstog than the other media for four of the six epiphytes tested. Germination was maximum on Knudson C medium with activated charcoal for four of the six terrestrial/lithophyte species. The results indicate a greater preference for nitrogen from amino acids rather than ammonium or nitrate salts in seeds of epiphytes compared with some terrestrial orchid species.

Full text available from: Official URL: http://www.frim.gov.my/v1/JTFSOnline/jtfs/v23n2/20...

Abstract

Although numerous media have been developed to evaluate both orchid seed germination and further seedling development after storage few studies have attempted to relate nutritional requirements to life history traits. For a range of terrestrial lithophytic and epiphytic orchid species comparisons were made of germination on Knudson C with and without activated charcoal or banana powder Norstog and Phytamax„ media to represent variations in available nitrogen. Germination varied with maximum values ranging from just 9 for Prosthechea cochleata Platanthera sp. and Spathoglottis paulinae to 95 for Phragmapedium longifolium. Along with Paphiopedilumdelenatii Paphiopedilum philippinense and the epiphyte Guarianthe bowringiana P. longifolium germinated well on most media. Germination was significantly higher on Norstog than the other media for four of the six epiphytes tested. Germination was maximum on Knudson C medium with activated charcoal for four of the six terrestrial/lithophyte species. The results indicate a greater preference for nitrogen from amino acids rather than ammonium or nitrate salts in seeds of epiphytes compared with some terrestrial orchid species.

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Item Type:	Article
AGROVOC Term:	Orchidaceae
AGROVOC Term:	Nitrogen
AGROVOC Term:	Seed germination
AGROVOC Term:	Seedlings
AGROVOC Term:	Nutritional requirements
AGROVOC Term:	Activated charcoal
AGROVOC Term:	Epiphytes
AGROVOC Term:	Seed collection
AGROVOC Term:	Relative humidity
AGROVOC Term:	Sterilization
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/21452

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