Assessment of genetic diversity on goat breeds in Malaysia using microsatellite markers

Amie Marini A.B., . and Hifzan R.M., . and Tan S.G., . and Panandam J.M., . Assessment of genetic diversity on goat breeds in Malaysia using microsatellite markers. pp. 19-26. ISSN 1394-3227

Genetic characterisation of goat populations in Malaysia is essential for conservation strategy as well as for genetic improvement. The goat populations in Malaysia comprise the small-framed original Katjang goats and the larger framed exotic goats: the Jamnapari Boer and Savanna. With continuous importation these exotic breeds are slowly replacing the indigenous Katjang which is in danger of extinction if there is no concrete plan to conserve the breed. The aim of this study was to determine the genetic diversity of goat breeds in Malaysia using microsatellite markers for proper utilisation in breeding program. Thirty microsatellite markers were used successfully to amplify caprine genomic DNA using a PCR-based technique. Low levels of number of alleles per locus were found in the Katjang Jamnapari Boer and Savanna breeds. The mean number of alleles per locus was 5.69. All four breeds and loci showed significant (p0.05) deviations from HWE except for three loci. The results of the FIS determinations together with the low number of alleles indicate the occurrence of inbreeding in all four breeds.

Full text available from: Official URL: http://www.msap.my/pdf/2-Assesment-Amie_rev6.pdf

Abstract

Genetic characterisation of goat populations in Malaysia is essential for conservation strategy as well as for genetic improvement. The goat populations in Malaysia comprise the small-framed original Katjang goats and the larger framed exotic goats: the Jamnapari Boer and Savanna. With continuous importation these exotic breeds are slowly replacing the indigenous Katjang which is in danger of extinction if there is no concrete plan to conserve the breed. The aim of this study was to determine the genetic diversity of goat breeds in Malaysia using microsatellite markers for proper utilisation in breeding program. Thirty microsatellite markers were used successfully to amplify caprine genomic DNA using a PCR-based technique. Low levels of number of alleles per locus were found in the Katjang Jamnapari Boer and Savanna breeds. The mean number of alleles per locus was 5.69. All four breeds and loci showed significant (p0.05) deviations from HWE except for three loci. The results of the FIS determinations together with the low number of alleles indicate the occurrence of inbreeding in all four breeds.

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Item Type:	Article
AGROVOC Term:	Goats
AGROVOC Term:	Breeding
AGROVOC Term:	Inbreeding
AGROVOC Term:	animal genetics
AGROVOC Term:	Microsatellites
AGROVOC Term:	Genetic resources
AGROVOC Term:	Genetic resources conservation
AGROVOC Term:	Genetic improvement
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	26 Apr 2025 14:37
URI:	http://webagris.upm.edu.my/id/eprint/21512

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