Comparison of bovine embryonic development of parthenogenetically activated oocytes by different combinations of chemical treatments

Siti Khadijah I., . and Rahman M. M., . and Abdullah R. B., . and Wan Khadijah W. E., . Comparison of bovine embryonic development of parthenogenetically activated oocytes by different combinations of chemical treatments. pp. 85-94. ISSN 1394-3227

Oocyte activation is the efficient method or step for successful animal cloning. This study was carried out to evaluate the embryonic development for both parthenotes and fertilised embryos according to the oocyte grades (Grades A B and C). Recovered oocytes according to the oocyte grades were divided into various combination treatments: (i) A231876-DMAP (ii) Iono6-DMAP (iii) EtOH6-DMAP (iv) IonoCHX and (v) IVF (control). Treatment with combination of 10 M Iono2 mM 6-DMAP indicated the highest embryonic development rates for 8-cell morula and blastocyst stages compared to the other combination treatments. There were significant differences between combination of Iono6-DMAP and IVF control at morula (24.3 vs. 3.4) and blastocyst stages (7.1 vs. 0.0) respectively. There was no consistent relationship between oocyte grades and embryo development rates. The data also showed that no differences were observed in blastocyst rates for each oocyte grade for all activation treatments including IVF control. In conclusion to activate bovine oocytes for later embryonic development stages (8-cell morula and blastocyst) the best combination treatment obtained in this study was the combination of 10 M Iono (5 min)2 mM 6-DMAP (4 h) regardless of the grades of oocytes including the importance of the optimal combination of chemical activation to produce bovine embryos in vitro.

Full text available from: Official URL: http://www.msap.my/pdf/8-Comparison-Ramli_rev7.pdf

Abstract

Oocyte activation is the efficient method or step for successful animal cloning. This study was carried out to evaluate the embryonic development for both parthenotes and fertilised embryos according to the oocyte grades (Grades A B and C). Recovered oocytes according to the oocyte grades were divided into various combination treatments: (i) A231876-DMAP (ii) Iono6-DMAP (iii) EtOH6-DMAP (iv) IonoCHX and (v) IVF (control). Treatment with combination of 10 M Iono2 mM 6-DMAP indicated the highest embryonic development rates for 8-cell morula and blastocyst stages compared to the other combination treatments. There were significant differences between combination of Iono6-DMAP and IVF control at morula (24.3 vs. 3.4) and blastocyst stages (7.1 vs. 0.0) respectively. There was no consistent relationship between oocyte grades and embryo development rates. The data also showed that no differences were observed in blastocyst rates for each oocyte grade for all activation treatments including IVF control. In conclusion to activate bovine oocytes for later embryonic development stages (8-cell morula and blastocyst) the best combination treatment obtained in this study was the combination of 10 M Iono (5 min)2 mM 6-DMAP (4 h) regardless of the grades of oocytes including the importance of the optimal combination of chemical activation to produce bovine embryos in vitro.

[error in script]

Item Type:	Article
AGROVOC Term:	Cloning
AGROVOC Term:	Parthenogenesis
AGROVOC Term:	Bovines
AGROVOC Term:	Oocytes
AGROVOC Term:	Chemical analysis (methods)
AGROVOC Term:	Embryonic development
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	26 Apr 2025 14:37
URI:	http://webagris.upm.edu.my/id/eprint/21519

Actions (login required)

View Item