Citation
Nadzirah K.Z., . and Zainal S., . and Noriham A., . and Normah I., . Efficacy of selected purification techniques for bromelain. pp. 43-46. ISSN 22317546
Abstract
Bromelain is one of the vegetal proteases found in pineapple plant. It has numerous applications in food and pharmaceuticals. This review discussed different bromelain purification techniques which will assist in determining the effect of processing conditions on the purification efficacy. There are four purification techniques to be discussed namely; reverse micellar system aqueous two phase extraction cation exchange chromatography and ammonium sulphate precipitation. Of the four techniques cation exchange chromatography had shown the best bromelain purification technique with purification fold of 10.0 followed by reverse micellar system containing CTAB/ isooctane/ hexanol/ butanol ATPE containing PEG polymer ammonium sulphate precipitation and ATPE containing PEO-PPO-PEO with purification fold of 5.2 4 2.81 and 1.25 respectively.
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Abstract
Bromelain is one of the vegetal proteases found in pineapple plant. It has numerous applications in food and pharmaceuticals. This review discussed different bromelain purification techniques which will assist in determining the effect of processing conditions on the purification efficacy. There are four purification techniques to be discussed namely; reverse micellar system aqueous two phase extraction cation exchange chromatography and ammonium sulphate precipitation. Of the four techniques cation exchange chromatography had shown the best bromelain purification technique with purification fold of 10.0 followed by reverse micellar system containing CTAB/ isooctane/ hexanol/ butanol ATPE containing PEG polymer ammonium sulphate precipitation and ATPE containing PEO-PPO-PEO with purification fold of 5.2 4 2.81 and 1.25 respectively.
Additional Metadata
| Item Type: | Article |
|---|---|
| AGROVOC Term: | Extraction |
| AGROVOC Term: | Chromatography |
| AGROVOC Term: | Cation exchange capacity |
| AGROVOC Term: | Ammonium sulphate |
| AGROVOC Term: | Precipitation |
| Depositing User: | Ms. Suzila Mohamad Kasim |
| Last Modified: | 24 Apr 2025 06:27 |
| URI: | http://webagris.upm.edu.my/id/eprint/21585 |
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