Isolation of lactic acid bacteria strain Staphylococcus piscifermentans from Malaysian traditional fermented shrimp cincaluk

Hajar S., . and Hamid T. H. T. A., . Isolation of lactic acid bacteria strain Staphylococcus piscifermentans from Malaysian traditional fermented shrimp cincaluk. pp. 125-129. ISSN 22317546

Lactic acid bacteria is well known for it uses as starter culture in various fermented food and it functions as a good natural antimicrobial agent. Cincaluk a Malaysian fermented shrimp product commonly found in traditional dishes is commonly enriched with LAB. Out of 50 colonies from a local cincaluk 7 strains were successfully isolated and shown to be positive in lactose utilization and catalase tests. The majority of the isolates from cincaluk showed Gram-positive cocci morphology and belonged to the group Staphyloccoccus spp. By using agar disc diffusion method the anti-bacterial properties of these isolates (namely isolate 1 2 3 4 5 6 and 7) moderately inhibited the growth of several pathogenic strains i.e. Escherichia coli Staphylococcus aureus Salmonella typhimurium and Bacillus subtilis which were used as indicator bacteria. Other than isolates 1 2 3 and 5; the 16S rRNA gene for isolate 6 and 7 were successfully amplified. The 16S rRNA gene fragment from isolate 7 was successfully cloned and sequenced. Based on rRNA sequences both isolates 6 and 7 belonged to the group Staphylococcus piscifermentans a rare strain previously reported to be specifically isolated exclusive from fish sources.

Full text available from: Official URL: http://ifrj.upm.edu.my/20%20(01)%202013/18%20IFRJ%...

Abstract

Lactic acid bacteria is well known for it uses as starter culture in various fermented food and it functions as a good natural antimicrobial agent. Cincaluk a Malaysian fermented shrimp product commonly found in traditional dishes is commonly enriched with LAB. Out of 50 colonies from a local cincaluk 7 strains were successfully isolated and shown to be positive in lactose utilization and catalase tests. The majority of the isolates from cincaluk showed Gram-positive cocci morphology and belonged to the group Staphyloccoccus spp. By using agar disc diffusion method the anti-bacterial properties of these isolates (namely isolate 1 2 3 4 5 6 and 7) moderately inhibited the growth of several pathogenic strains i.e. Escherichia coli Staphylococcus aureus Salmonella typhimurium and Bacillus subtilis which were used as indicator bacteria. Other than isolates 1 2 3 and 5; the 16S rRNA gene for isolate 6 and 7 were successfully amplified. The 16S rRNA gene fragment from isolate 7 was successfully cloned and sequenced. Based on rRNA sequences both isolates 6 and 7 belonged to the group Staphylococcus piscifermentans a rare strain previously reported to be specifically isolated exclusive from fish sources.

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Item Type:	Article
AGROVOC Term:	Lactic acid bacteria
AGROVOC Term:	Staphylococcus
AGROVOC Term:	Prawns and shrimps
AGROVOC Term:	Fermented products
AGROVOC Term:	Fermented foods
Geographical Term:	Malaysia
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	26 Apr 2025 15:09
URI:	http://webagris.upm.edu.my/id/eprint/21597

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