Free radical scavenging activity of Sargassum siliquosum J. G. Agardh

Corpuz M. J. A. T., . and Osi M. O., . and Santiago L. A., . Free radical scavenging activity of Sargassum siliquosum J. G. Agardh. pp. 291-297. ISSN 22317546

The ability of Sargassum siliquosum to prevent the initiation of free radicals to cause cellular damage was investigated in vitro. The plant was extracted exhaustively using methanol and partition using solvents of different polarities. Total phenolic contents (TPC) and flavonoid contents (TFC) were evaluated according to Folin-Ciocalteu and aluminium chloride colorimetric assays respectively. TPC results showed significant difference in the mean gallic acid equivalent (GAE) (p0.001) using the five fractions: water (butanol-water methanol-water) hexane dichloromethane (DCM) in which the mean GAE using DCM is significantly the highest with 69.03 mg/g GAE. TFC demonstrated a significant difference in the mean quercitin equivalent (p0.001) in which the mean QE using dichloromethane is significantly the highest (65 mg/g) compared with other samples. Radical scavenging activities of fractions with the highest TPC and TFC were tested against OH NO and H2O2. The strongest inhibition was demonstrated by DCM fraction against radical scavengers OH NO and H2O2 in a dose-dependent manner with IC50 value of 0.28 0.29 and 2.27 mg/mL respectively. These results clearly indicate the beneficial effect of S. siliquosum as antioxidant and anticancer agent being a free radical scavenger.

Full text available from: Official URL: http://ifrj.upm.edu.my/20%20(01)%202013/39%20IFRJ%...

Abstract

The ability of Sargassum siliquosum to prevent the initiation of free radicals to cause cellular damage was investigated in vitro. The plant was extracted exhaustively using methanol and partition using solvents of different polarities. Total phenolic contents (TPC) and flavonoid contents (TFC) were evaluated according to Folin-Ciocalteu and aluminium chloride colorimetric assays respectively. TPC results showed significant difference in the mean gallic acid equivalent (GAE) (p0.001) using the five fractions: water (butanol-water methanol-water) hexane dichloromethane (DCM) in which the mean GAE using DCM is significantly the highest with 69.03 mg/g GAE. TFC demonstrated a significant difference in the mean quercitin equivalent (p0.001) in which the mean QE using dichloromethane is significantly the highest (65 mg/g) compared with other samples. Radical scavenging activities of fractions with the highest TPC and TFC were tested against OH NO and H2O2. The strongest inhibition was demonstrated by DCM fraction against radical scavengers OH NO and H2O2 in a dose-dependent manner with IC50 value of 0.28 0.29 and 2.27 mg/mL respectively. These results clearly indicate the beneficial effect of S. siliquosum as antioxidant and anticancer agent being a free radical scavenger.

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Item Type:	Article
AGROVOC Term:	Sargassum
AGROVOC Term:	Hydroxylamine
AGROVOC Term:	Hydrogen peroxide
AGROVOC Term:	Nitric oxide
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/21618

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