Application of PCR and MAS: potential use for assessment of genetic diversity of rice germplasm in breeding programmes in developing countries

Wijerathna Y. M. A. M., . and Perera A. N. K., . and Hamama I. B., . and Hoang L., . Application of PCR and MAS: potential use for assessment of genetic diversity of rice germplasm in breeding programmes in developing countries. pp. 161-174. ISSN 1511-3701

Molecular characterisations of genotypes give precise information about the extent of genetic diversity which assists in the development of an appropriate rice breeding programme. The latest approach in plant biotechnology and molecular breeding which is the development of the polymerase chain reaction (PCR) for amplifying DNA DNA sequencing and data analysis is an effective technique that can be used for the screening characterisation and evaluation of genetic diversity. Traits that serve as genetic markers are by definition polymorphic; the more polymorphic the trait the greater its potential value to germplasm management. The issue of homology may seem insignificant for morphological markers but the increasing use of molecular markers has heightened its importance. Application of molecular markers is still prohibitively expensive for most large-scale applications in rice breeding programmes where performance parameters such as yield quality disease resistance and other desirable growth characteristics are upgraded. Therefore marker assisted selection (MAS) methods are currently used for more targeted applications in order to keep up with the rising demand for rice consumption. Since conventional breeding methods will not be able to meet the satisfactory harvest the application of biotechnological tools is one plausible option to tap into the significant yield potential of rice.

Full text available from: Official URL: http://pertanika/Pertanika%20PAPERS/JTAS%20Vol.%20...

Abstract

Molecular characterisations of genotypes give precise information about the extent of genetic diversity which assists in the development of an appropriate rice breeding programme. The latest approach in plant biotechnology and molecular breeding which is the development of the polymerase chain reaction (PCR) for amplifying DNA DNA sequencing and data analysis is an effective technique that can be used for the screening characterisation and evaluation of genetic diversity. Traits that serve as genetic markers are by definition polymorphic; the more polymorphic the trait the greater its potential value to germplasm management. The issue of homology may seem insignificant for morphological markers but the increasing use of molecular markers has heightened its importance. Application of molecular markers is still prohibitively expensive for most large-scale applications in rice breeding programmes where performance parameters such as yield quality disease resistance and other desirable growth characteristics are upgraded. Therefore marker assisted selection (MAS) methods are currently used for more targeted applications in order to keep up with the rising demand for rice consumption. Since conventional breeding methods will not be able to meet the satisfactory harvest the application of biotechnological tools is one plausible option to tap into the significant yield potential of rice.

[error in script]

Item Type:	Article
AGROVOC Term:	PCR
AGROVOC Term:	Genetic diversity within species
AGROVOC Term:	Rice
AGROVOC Term:	Germplasm
AGROVOC Term:	Breeding methods
AGROVOC Term:	Developing countries
AGROVOC Term:	Genotypes
AGROVOC Term:	Plant biotechnology
AGROVOC Term:	DNA
AGROVOC Term:	RNA
Depositing User:	Ms. Suzila Mohamad Kasim
Last Modified:	24 Apr 2025 06:27
URI:	http://webagris.upm.edu.my/id/eprint/21638

Actions (login required)

View Item